Exploration of Cyclooxygenase-2 (COX-2) Inhibitory Peptides from Walnut Dreg Proteins Based on In Silico and In Vitro Assessments
作者机构：College of Food Science and Technology， Yunnan Agricultural University Puer University Key Laboratory of Puer Tea Science， Ministry of Education， Yunnan Agricultural University Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources， Ministry of Education， Yunnan Agricultural University Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food， Yunnan Agricultural University National Research and Development Professional Center for Moringa Processing Technology， Yunnan Agricultural University
出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康(英文))
年 卷 期：2023年
核心收录：
学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1007[医学-药学(可授医学、理学学位)] 08[工学] 083202[工学-粮食、油脂及植物蛋白工程] 10[医学]
基 金：supported by the Major Project of Science and Technology Department of Yunnan Province (202002AA100005 and 202102AE090027-2) the YEFICRC project of Yunnan provincial key programs (2019ZG009) Yunnan Province Ten Thousand Plan Industrial Technology Talents project (YNWR-CYJS-2020-010) the Yunnan Provincial Department of Science and Technology Agricultural Joint Special Project (202101BD070001-120)
摘 要：Walnut dreg protein hydrolysates (WDPHs) exhibit a variety of biological activities， however， the COX-2 inhibitory peptide of WDPHs remain unclear. This aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis. In total， 1262 peptide sequences were observed by nano LC-MS/MS and four novel COX-2 inhibitory peptides (AGFP， FPGA， LFPD， and VGFP) were identified for the first time. Enzyme kinetic data indicated that AGFP， FPGA， and LFPD displayed mixed-type COX-2 inhibition， whereas VGFP was a non-competitive inhibitor. This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site. These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.
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