Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier

作     者：Yang Liu Qing Liu Chengcheng Zhang Jianxin Zhao Hao Zhang Wei Chen Qixiao Zhai Yang Liu;Qing Liu;Chengcheng Zhang;Jianxin Zhao;Hao Zhang;Wei Chen;Qixiao Zhai

作者机构：State Key Laboratory of Food Science and TechnologySchool of Food Science and TechnologyJiangnan UniversityWuxi 214122China National Engineering Research Center for Functional FoodJiangnan UniversityWuxi 214122China Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi BranchWuxi 214122China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2023年第12卷第5期

页      面：1526-1537页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the Natural Science Foundation of Jiangsu Province (BK20200084) The National Natural Science Foundation of China (32021005 and 31871773) Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。 

主　　题：Akkermansia muciniphila Intestinal barrier Strain specificity Comparative genomics Bacterial surface components 

摘      要：Akkermansia muciniphila, one of the most promising next-generation probiotics, was reported to exhibit beneficial modulatory effects on the gut barrier. However, the strain-specific and underlying regulatory mechanisms of this species on gut barrier function were not well studied. Therefore, this study evaluated the protective effect of A. muciniphila strains on the intestinal barrier and investigated the mode of action and material basis of this modulatory effect. We first confirmed the strain-specific effects of A. muciniphila on intestinal barrier regulation and found that this phenomenon may be explained by the different abilities of strains to affect tight junction protein expression in enterocytes. Comparative genomic analysis proved that the ability of A. muciniphila to regulate the intestinal barrier was exerted in part by the functional genes(such as COG0438, COG0463, and COG2244)related to the synthesis of cellular surface proteins. The role of these surface proteins in intestinal barrier regulation was further verified by strain-comparative experiments in animal and cell models and surface protein removal trials. This study confirmed the different effects of A. muciniphila strains on gut barrier modulation and provided molecular and genetic targets for the screening of A. muciniphila strains with superior protection against gut barrier dysfunction.