Cloning and Alternative Splicing Analysis of Bombyx mori Transformer-2 Gene using Silkworm EST Database

作     者：Bao-Long NIU Zhi-Qi MENG Yue-Zhi TAO Shun-Lin LU Hong-Biao WENG Li-Hua HE Wei-Feng SHEN Sericultural Research Institute,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China Department of Sericulture and Apiculture,College of Animal Sciences,Zhejiang University,Hangzhou 310029,China 

作者机构：Sericultural Research Institute Zhejiang Academy of Agricultural SciencesHangzhou 310021 China Department of Sericulture and Apiculture College of Animal Sciences Zhejiang UniversityHangzhou 310029 China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2005年第37卷第11期

页      面：728-736页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：EST database transformer-2 gene Bmtra-2 alternative splicing gene clone Bombyxmori 

摘      要：We have identified Bombyx mori transformer-2 gene(Bmtra-2)cDNA by blasting the ESTdatabase of *** was expressed in the whole life of the male and female silkworm and was observed asa band of 1.3 kb by Northern blot *** comparing corresponding ESTs to the Bmtra-2 DNA sequence,it was revealed that there were eight exons and seven introns,and all splice sites of exons/introns conformedto the GT/AG ***-2 pre-mRNA can produce multiple mRNAs encoding six distinct isoforms ofBmTRA-2 protein using an alternative splicing pathway during *** types of Bmtra-2 cDNAclones were identified by reverse transcription-polymerase chain *** isoforms of BmTRA-2 proteincontain two arginine/serine-rich domains and one RNA recognition motif,showing striking organizationalsimilarity to Drosophila TRA-2 proteins.