Molecularly imprinted polymer for pre-concentration of esculetin from tobacco followed by the UPLC analysis

作     者：RAZWAN SARDAR M. JIN Yan KONG Guang Hui LI Hai Fang LIN Jin-Ming 

作者机构：Department of Chemistry Beijing Key Laboratory of Microanalytical Methods and Instrumentation Tsinghua University Nova Med Pharmaceuticals Pvt. Ltd. Yunnan Academy of Tobacco Agricultural Science 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2014年第57卷第12期

页      面：1751-1759,1-2页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：supported by the Yunnan Green Tobacco Production Research(2011YN01) the China Equipment and Education Resources System(CERS-1-75) 

主　　题：分子印迹聚合物 预浓缩 7-甲氧基香豆素 4-乙烯基吡啶 二甲基丙烯酸酯 扫描电子显微镜 烟草 模板分子 

摘      要：Molecularly imprinted polymers(MIPs)for solid-phase extraction and pre-concentration of esculetin have been successfully prepared by the bulk polymerization method using esculetin as a template *** of varying composition were prepared using different monomers(4-vinylpyridine,methacrylic acid,and acrylamide),ethylene glycol dimethacrylate as the cross-linker,2,2-azobis(2-methylpropinitrile)as the initiator,and different porogen solvents(ethanol,acetone/methanol,and acetonitrile).The best polymer was obtained when 4-vinylpyridine was used as the monomer and acetone/methanol(3:2)as the porogen solvent,whereas the template:-monomer:-cross-linker ratio was 1:4:*** imprinting factor of the selected MIPs for esculetin was *** polymers were evaluated according to their selective recognition properties for esculetin and structurally-related compounds(esculin,scopoletin,coumarin,and 7-methoxycoumarin).Chemical and morphological characterizations of the polymers were investigated by FTIR and scanning electron microscope,which confirmed a high degree of *** area,pore volume,and pore size of the polymer were investigated by Brunauer-Emmett-Teller *** were also successfully used as solid-phase adsorbent materials for the extraction of esculetin from tobacco *** contents in dried tobacco leaves were found to be(9.27±0.17)μg g-1.