Nanoprobes for super-resolution fluorescence imaging at the nanoscale

作     者：HOU ShangGuo LIANG Le DENG SuHui CHEN JianFang HUANG Qing CHENG Ya FAN ChunHai 

作者机构：Division of Physical Biology and Bioimaging CenterShanghai Synchrotron Radiation Facility Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai Institute of Optics and Fine MechanicsChinese Academy of Sciences 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2014年第57卷第1期

页      面：100-106页

核心收录：

学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：supported by the National Basic Research Pro-gram of China(973 program,2012CB825805,2013CB933800,2012CB-932603) the National Natural Science Foundation of China(10975179,61008056,and 61078016) 

主　　题：super-resolution microscopy,diffraction barrier,switching characteristic 

摘      要：Compared with other imaging techniques,fluorescence microscopy has become an essential tool to study cell biology due to its high compatibility with living *** to the resolution limit set by the diffraction of light,fluorescence microscopy could not resolve the nanostructures in the range of200 ***,many techniques have been emerged to overcome the diffraction barrier,providing nanometer spatial *** the course of development,the progress in fluorescent probes has helped to promote the development of the high-resolution fluorescence ***,we describe the contributions of the fluorescent probes to far-field super resolution imaging,focusing on concepts of the existing super-resolution nanoscopy based on the photophysics of fluorescent nanoprobes,like photoswitching,bleaching and *** probe technology is crucial in the design and implementation of super-resolution imaging methods.