Subtype and sequence analysis of HIV-1 strains in Heilongjiang Province

作     者：WANG Fu-xiang ZHOU Hui LING Hong ZHOU Hai-zhou LIU Wei-hua SHAO Yi-ming ZHOU Jin 

作者机构：Department of Infectious Diseases First Clinical College of Harbin Medical University Harbin 150001 China Department of Hematology First Clinical College of Harbin Medical University Harbin 150001 China Department of Microbiology Harbin Medical University Harbin 150081 China Agriculture General Hospital Harbin 150001 China National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100050 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2007年第120卷第22期

页      面：2006-2010页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：This work was supported by the grants from the Heilongjiang Province Postdoctor Foundation (No. LRB-OS-263) the Board of Health Foundation of Heilongjiang Province (No. 2005-172) the Foundation of the First Clinical College of the Harbin Medical University 

主　　题：human immunodeficiency virus type 1 sequence analysis 

摘      要：Background Human immunodeficiency virus （HIV-1） is divided into two types, HIV-1 （groups M, N and O） and HIV-2. Heilongjiang Province located in the northeast of China, and the feature of the subtype distribution and sequence characteristics of HIV-1 strains prevalent in Heilongjiang Province is still uncertain. The aim of this study was to investigate the subtype distribution and genetic characteristics of HIV-1 strains in one hospital in Heilongjiang Province. Methods HIV-1 env gene was amplified by nested-PCR from peripheral blood mononuclear cells （PBMCs） obtained from 19 HIV-1 seropositive individuals in Heilongjiang Province. The C2-V3 region was sequenced. Aligned the nucleotide sequence of 19 samples with CLUSTAL W （BioEdit） software, results were acquired and used for phylogenetic tree analysis after artificial adjustment. Reference sequence, downloaded from Los Alamos HIV Sequence Database, was used to identify the subtype of obtained sequence. Genetic distance between sequences was assessed using the software MEGA 3.1 Kimura 2-parameter, and the Phylogenetic tree was reestablished with Neighbor-Joining method. Results Phylogenetic tree analysis showed that 19 Heilongjiang strains clustered closely to subtype B strain from Thailand and were far from other international subtype reference strains. Statistical test showed no significant discrepancy between the genetic distance of interclass and intra-class （P〉0.05）. The analysis of V3 loop amino sequence of 19 Heilongjiang B strains revealed that V3 tip motif of 10 samples （52.63%） was GPGQ, and of 4 samples （21.53%） was GPGR. Conclusions The subtype of 19 HIV-1 seropositive individuals in Heilongjiang Province is B′, and it is introduced from He＇nan Province. V3 tip motifs of the HIV-1 isolates are mainly GPGQ and GPGR.