Overexpression of auxin/indole-3-acetic acid gene MdIAA24 enhances Glomerella leaf spot resistance in apple(Malus domestica)

作     者：Qian Wang Dong Huang Wenyan Tu Fengwang Ma Changhai Liu Qian Wang;Dong Huang;Wenyan Tu;Fengwang Ma;Changhai Liu

作者机构：State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of AppleCollege of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2024年第10卷第1期

页      面：15-24页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 0902[农学-园艺学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the National Key Research and Development Program of China(Grant No.2018YFD1000307) the National Natural Science Foundation of China(Grant No.32172529) the Special Funds for Major Scientific and Technological Innovation from Shaanxi Province(Grant No.2020zdzx03-0101) the Earmarked Fund for China Agriculture Research System(Grant No.CARS-27) China Postdoctoral Science Foundation(Grant Nos.2017M610657,2018T111108) 

主　　题：Apple MdIAA24 Glomerella leaf spot(GLS) Antioxidant capacity Auxin Salicylic acid Jasmonic acid 

摘      要：Auxin is throughout the entire life process of plants and is involved in the crosstalk with other hormones,yet its role in apple disease resistance remains *** this study,we investigated the function of auxin/indole-3-acetic acid(IAA)gene Md IAA24 overexpression in enhancing apple resistance to Glomerella leaf spot(GLS)caused by Colletotrichum fructicola(Cf).Analysis revealed that,upon Cf infection,35S::Md IAA24 plants exhibited enhanced superoxide dismutase(SOD)and peroxidase(POD)activity,as well as a greater amount of glutathione(reduced form)and ascorbic acid accumulation,resulting in less H_(2)O_(2)and superoxide anion(O_(2)^(-))in apple ***,35S::Md IAA24 plants produced more protocatechuic acid,proanthocyanidins B1,proanthocyanidins B2 and chlorogenic acid when infected with *** Cf infection,35S::Md IAA24 plants presented lower levels of IAA and jasmonic acid(JA),but higher levels of salicylic acid(SA),along with the expression of related *** overexpression of Md IAA24 was observed to enhance the activity of chitinase andβ-1,3-glucanase in Cfinfected *** results indicated the ability of Md IAA24 to regulate the crosstalk between IAA,JA and SA,and to improve reactive oxygen species(ROS)scavenging and defense-related enzymes *** jointly contributed to GLS resistance in apple.