Physical exercise suppresses hepatocellular carcinoma progression by alleviating hypoxia and attenuating cancer stemness through the Akt/GSK-3β/β-catenin pathway

作     者：Chu-lan Xiao Zhi-peng Zhong Can Lü Bing-jie Guo Jiao-jiao Chen Tong Zhao Zi-fei Yin Bai Li Chu-lan Xiao;Zhi-peng Zhong;Can Lü;Bing-jie Guo;Jiao-jiao Chen;Tong Zhao;Zi-fei Yin;Bai Li

作者机构：Department of RehabilitationChanghai HospitalNaval Medical UniversityShanghai 200433China Department of Traditional Chinese MedicineThe 920th Hospital of Joint Logistics Support ForceKunming 650000Yunnan ProvinceChina Second TeamGraduate SchoolNaval Medical UniversityShanghai 200433China College of Integrated Traditional Chinese and Western MedicineNanjing University of Traditional Chinese MedicineNanjing 210023Jiangsu ProvinceChina Department of Military Traditional Chinese MedicineFaculty of Traditional Chinese MedicineNaval Medical UniversityShanghai 200433China 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2023年第21卷第2期

页      面：184-193页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.82104977) Youth Start-up Foundation of Changhai Hospital(No.2019QNB05) 

主　　题：Physical exercise Swimming Hepatocellular carcinoma Liver cancer Blood perfusion Hypoxia Cancer stemness Akt/GSK-3β/β-catenin 

摘      要：Objective:Physical exercise,a common non-drug intervention,is an important strategy in cancer treatment,including hepatocellular carcinoma(HCC).However,the mechanism remains largely *** to the importance of hypoxia and cancer stemness in the development of HCC,the present study investigated whether the anti-HCC effect of physical exercise is related to its suppression on hypoxia and cancer ***:A physical exercise intervention of swimming(30 min/d,5 d/week,for 4 weeks)was administered to BALB/c nude mice bearing subcutaneous human HCC *** anti-HCC effect of swimming was assessed in vivo by tumor weight monitoring,hematoxylin and eosin(HE)staining,and immunohistochemistry(IHC)detection of proliferating cell nuclear antigen(PCNA)and *** expression of stemness transcription factors,including Nanog homeobox(NANOG),octamer-binding transcription factor 4(OCT-4),v-Myc avian myelocytomatosis viral oncogene homolog(C-MYC)and hypoxia-inducible factor-1a(HIF-1a),was detected using real-time reverse transcription polymerase chain reaction.A hypoxia probe was used to explore the intratumoral hypoxia *** blot was used to detect the expression of HIF-1a and proteins related to protein kinase B(Akt)/glycogen synthase kinase-3β(GSK-3β)/β-catenin signaling *** IHC analysis of platelet endothelial cell adhesion molecule-1(CD31),and the immunofluorescence co-location of CD31 and desmin were used to analyze tumor blood ***-7721 cells were treated with nude mice *** inhibition effect on cancer stemness in vitro was detected using suspension sphere experiments and the expression of stemness transcription *** hypoxia status was inferred by measuring the protein and mRNA levels of ***,the expression of proteins related to Akt/GSK-3β/β-catenin signaling pathway was ***:Swimming significantly reduced the body weight and tumor weight in nude mice bearing HCC *** staining and IHC results show