Correlation of gene content in selected bifidobacteria with folate supplier or scavenger status during growth in laboratory media

作     者：Omer F.Celik Daniel J.O’Sullivan 

作者机构：Department of Food Science and NutritionMicrobial and Plant Genomics InstituteUniversity of MinnesotaSt.PaulMinnesotaUSA Department of Food EngineeringFaculty of AgricultureOrdu University52200OrduTurkey 

出 版 物：《Food Bioscience》 (食品生物科学（英文）)

年 卷 期：2023年第51卷第1期

页      面：1057-1066页

核心收录：

学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学] 

基　　金：Center for Mass Spectrometry and Proteomics 

主　　题：Folic acid PABA Probiotics Folate 

摘      要：Five different bifidobacterial cultures were screened to investigate if their de novo folate production ability could be predicated based on their folate biosynthetic gene *** previously developed folate free media could not adequately support the growth of these five strains,even with added folate,an alternative medium(YNB+)was *** with its genome prediction,Bifidobacterium animalis *** strain Bb-12 required folate for its growth even in the presence of p-aminobenzoic acid(PABA).Although *** DJO10A and *** ATCC 15701 lack an alkaline phosphatase gene for the formation of one pathway intermediate(DHPPP),both were found to be able to grow independent from folate and PABA suggesting that lack of these genes in genome annotations cannot be used to predict folate dependency in ***,having all required genes cannot always predict folate production,as *** *** RECb4 contained a full set of pathway genes,but required folate or PABA for *** many bifidobacteria can produce folate,this also cannot be used to predicate their ability to supply folate as some can act as scavengers when folate is readily available in the environment,such as *** ATCC 15701 in this *** bifidobacterial cultures are sometimes considered for use in foods based on their folate production abilities,it is important to consider whether they function as folate scavengers or suppliers when growing in folate containing media.