Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment
作者机构：Department of OphthalmologyXiaoxian People’s HospitalSuzhou 234000Anhui ProvinceChina Graduate School of Bengbu Medical CollegeBengbu233000Anhui ProvinceChina Department of OphthalmologyEye Centerthe First AffiliatedHospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001Anhui ProvinceChina Graduate School of Anhui Medical UniversityHefei 230001Anhui ProvinceChina Hefei Aier Eye HospitalHefei 230001Anhui ProvinceChina
出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)
年 卷 期：2023年第16卷第3期
页 面：348-353页
核心收录：
学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学]
基 金：Supported by the National Natural Science Foundation of China(No.82070977)
主 题：autophagy-related proteins reactive oxygen species vitreous body retinal detachment
摘 要：AIM:To detect the concentrations of reactive oxygen species(ROS),transient receptor potential mucin-1(TRPML1),and autophagy-related(Atg)proteins(LC3-Ⅰ,LC3-Ⅱ,and Beclin1)in vitreous humor of patients with simple rhegmatogenous retinal detachment(RRD).METHODS:RRD patients enrolled as the RRD group,and patients with idiopathic macular hole(IMH)and idiopathic macular epiretinal membrane(IMEM)were enrolled as control *** levels of ROS,TRPML1,LC3-Ⅰ,LC3-Ⅱ,and Beclin1 in vitreous humor of patients in the RRD and control groups were detected by enzyme-linked immunosorbent assay(ELISA).RESULTS:The RRD group included 28 eyes 28 patients and had a higher concentration of ROS in vitreous humor(631.86±18.05 vs 436.34±108.22 IU/m L,P0.05).The ROS level in patients with a wide retinal detachment(RD)extent(RD range≥1/2)was higher than that with a narrow RD extent(RD range1/2,P0.05).ROS concentration was negatively correlated with RD time(r=-0.46,P=0.01).The expression levels of LC3-Ⅰand Beclin1 significantly decreased in RRD(P0.05),but there were no correlations with the RD time,RD extent,or macular ***:In eyes with RRD,the concentration of ROS in vitreous humor increases and the expression levels of Atg proteins decrease,reflecting possibly that autophagy is inhibited.
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