G2-LIKE CAROTENOID REGULATOR(SlGCR)is a positive regulator of lutein biosynthesis in tomato
作者机构：Key Laboratory of Bio-Resource and Eco-Environment of Ministry of EducationCollege of Life SciencesSichuan UniversityChengdu610064China Sanya Institute of China Agricultural UniversitySanya572025China Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable CropsCollege of HorticultureChina Agricultural UniversityBeijing100193China
出 版 物：《aBIOTECH》 (生物技术通报（英文版）)
年 卷 期：2022年第3卷第4期
页 面：267-280页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 0836[工学-生物工程] 090202[农学-蔬菜学]
基 金：funded by the Sichuan Science and Technology Program(2021YFYZ0027) the National Natural Science Foundation of China(32170266) the Institutional Research Fund of Sichuan University(2020SCUNL106) the Fundamental Research Funds for the Central Universities(SCU2022D003).We acknowledge the Mass Spectrometry Core Facility in the College of Life Sciences,Sichuan University,for assistance in metabolic analysis.
主 题：Solanum lycopersicum GARP G2-like transcription factor Lutein biosynthesis
摘 要：Lutein is an oxygen-containing carotenoid synthesized in plant chloroplasts and chromoplasts.It plays an indispensable role in promoting plant growth and maintaining eye health in humans.The rate-limiting step of lutein biosynthesis is catalyzed by the lycopeneε-cyclase enzyme(LCYE).Although great progress has been made in the identification of transcription factors involved in the lutein biosynthetic pathway,many systematic molecular mechanisms remain to be elucidated.Here,using co-expression analysis,we identified a gene,G2-LIKE CAROTENOID REGULATOR(SlGCR),encoding a GARP G2-like transcription factor,as the potential regulator of SlLCYE in tomato.Silencing of SlGCR reduced the expression of carotenoid biosynthetic genes and the accumulation of carotenoids in tomato leaves.By contrast,overexpression of SlGCR in tomato fruit significantly increased the expression of relevant genes and enhanced the accumulation of carotenoids.SlGCR can directly bind to the SlLCYE promoter and activate its expression.In addition,we also discovered that expression of SlGCR was negatively regulated by the master regulator SlRIN,thereby inhibiting lutein synthesis during tomato fruit ripening.Taken together,we identified SlGCR as a novel regulator involved in tomato lutein biosynthesis,elucidated the regulatory mechanism,and provided a potential tool for tomato lutein metabolic engineering.
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