Functional conservation and divergence of miR156 and miR529 during rice development

作     者：Yan Li Yizhou He Tian Qin Xuelian Guo Kun Xu Chunxue Xu Wenya Yuan Yan Li;Yizhou He;Tian Qin;Xuelian Guo;Kun Xu;Chunxue Xu;Wenya Yuan

作者机构：State Key Laboratory of Biocatalysis and Enzyme EngineeringHubei Collaborative Innovation Center for Green Transformation of Bio-ResourcesSchool of Life SciencesHubei UniversityWuhan 430062HubeiChina Oil Crops Research Institute of Chinese Academy of Agricultural SciencesWuhan 430062HubeiChina Wuhan City Vocational CollegeWuhan 430064HubeiChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2023年第11卷第3期

页      面：692-703页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(32101746,31872811) Hubei Provincial Natural Science Foundation of China(2021CFB033,2022CFB393) 

主　　题：MiRNA OsSPL14 Tiller number Grain size Oryza sativa 

摘      要：MicroRNAs(miRNAs)are important regulatory elements involved in the regulation of various plant developmental and physiological processes by blocking the expression of target ***156 and miR529 are two combinatorial regulators,which cooperatively target the SQUAMOSA PROMOTER BINDING-LIKE(SPL)family ***,there has been no report about the functional conservation and divergence of miR156 and miR529 during plant development to *** this study,the biological function and relationship of miR156,miR529 and their target Os SPL14 in rice were *** of miR156e or miR529a(miR156e-OE and miR529a-OE)increased the grain size and tiller number but decreased the plant height and panicle length,while an opposite phenotype was observed for their target mimicry(miR156-MIMIC and miR529a-MIMIC)transgenic ***-loop RT-PCR results revealed ubiquitous expression of miR156 in roots,axillary buds and leaves,while miR529 was preferentially expressed in the ***,Os SPL14 could be preferentially and precisely cleaved by miR529a in young panicle but by miR156 in vegetative *** plants generated by the target immune strategy exhibited obvious growth defects upon the blocking of miR156 and/or miR529 function in rice,confirming that both miR156 and miR529 play important roles in controlling rice growth and ***,the miR156/miR529-Os SPL14 module negatively controlled grain size by regulating the genes associated with grain size and cell cycling,and controlled plant height through a more complicated *** together,our results demonstrate that miR156 and miR529 respectively function dominantly in the vegetative stage and reproductive stage to control rice growth and development by regulating the accumulation of Os *** findings facilitate a better understanding of the functional conservation and divergence of miR156 and miR529 family in the miRNA combinatorial regulatory network of plants.