Pathogenicity and risk of cross-species transmission of Aerococcus viridans isolated from Tian-e-zhou National Reserve for Lipotes vexillifer in Shishou,China

作     者：Miaomiao Wu Yihui Fan Cheng Gong Xin Chen Lijuan Li Junfa Yuan 

作者机构：Department of Aquatic Animal MedicineCollege of FisheriesHuazhong Agricultural UniversityWuhan430070China Hubei Engineering Research Center for Aquatic Animal Diseases Control and PreventionWuhan430070China National Aquatic Animal Diseases Para-reference Laboratory(HZAU)Wuhan430070China Tian-e-zhou National Reserve for Lipotes VexilliferShishou434400China 

出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)

年 卷 期：2024年第9卷第5期

页      面：776-784页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Ocean Park Conservation Foundation, Hong Kong, OPCFHK, (MM01.2223) Ocean Park Conservation Foundation, Hong Kong, OPCFHK 

主　　题：Yangtze finless porpoise Aerococcus viridans Pathogenicity Cross-species transmission Mice Pelteobagrus fulvidraco 

摘      要：During the survey of potential pathogens in the Tian-e-zhou National Reserve for Lipotes vexillifer in Shishou,China,isolate SS20210504 was isolated from the diseased prey fish-Pelteobagrus fulvidraco and was identified as Aerococcus viridans through 16S rRNA gene,morphological observation,and physiological and biochemical *** infection trials with fish through different methods were *** indicated that the isolate of SS2021504 caused mortality in fish with the LD50 of 1.0×106.2 CFU/mL,1.0×107.9 CFU/mL and 1.0×106.8 CFU/mL with the infection method of intraperitoneal injection,co-immersion,and scratch immersion,*** fish exhibited hemorrhages signs similar to naturally diseased *** analysis showed that the liver and kidney were the principal target organs of isolate SS2021504 in fish,and obvious vacuolation and karyopyknosis occurred in the above *** evaluate the pathogenicity of isolate SS20210504 in mice,healthy mice and immunosuppressed mice were further experimentally infected with bacteria,*** death was observed either in healthy mice or in immunocompromised ***,the immunocompromised mice inoculated with bacteria showed obvious clinical symptoms and severe damage to multiple *** inflammatory signaling pathways were significantly activated with upregulated levels of Myd88,TNFα,AP-1,IL-1β,IL-6,and IFN-γgene in the spleen from the immunocompromised mice after infected with isolate *** findings imply that isolate SS20210504 has the potential risk to YFPs and *** surveillance of *** and other pathogens in the TZO reserve is suggested to prevent outbreaks in prey fish,YFPs,and residents as well.