Steroids and dihydroisocoumarin glycosides from Xylaria *** the one strain many compounds strategy and their bioactivities

作     者：GAN Dong LI Chenzhe SHU Yan WANG Jiapeng WANG Chengyao ZHU Li YANG Yujun LIU Jiaqi HE Bijian CAI Le DING Zhongtao GAN Dong;LI Chenzhe;SHU Yan;WANG Jiapeng;WANG Chengyao;ZHU Li;YANG Yujun;LIU Jiaqi;HE Bijian;CAI Le;DING Zhongtao

作者机构：Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan ProvinceKey Laboratory of Medicinal Chemistry for Natural ResourceMinistry of EducationSchool of Chemical Science and TechnologyYunnan UniversityKunming 650091China College of PharmacyDali UniversityDali 671003China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2023年第21卷第2期

页      面：154-160页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Xylaria sp. OSMAC strategy Secondary metabolite Bioactivity 

摘      要：The fungus Xylaria ***-15 was isolated from Illigera *** on the one strain many compounds(OSMAC)strategy,the strain was fermented on potato and rice solid media,*** a result,two novel steroids,xylarsteroids A(1)and B(2),which are the first examples of C28-steroid with an unusualβ-andγ-lactone ring,respectively,along with two new dihydroisocoumarin glycosides,xylarglycosides A(3)and B(4),were *** structures were elucidated by spectroscopic methods,X-ray diffraction and electronic circular dichroism(ECD)*** isolated compounds were evaluated for cytotoxicity,DPPH radical scavenging activity,acetylcholinesterase inhibitory and antimicrobial *** 1 exhibited potent AChE inhibitory activity with an IC50 value of 2.61±0.05μmol·L^(−1).Theβ-lactone ring unit of 1 is critical for its AChE inhibitory *** finding was further confirmed through exploring the interaction of 1 with AChE by molecular *** addition,both compounds 1 and 2 exhibited obvious antibacterial activity against Bacillus subtilis with a minimum inhibitory concentration(MIC)of 2μg·mL^(−1).Compounds 3 and 4 exhibited antibacterial activities against Staphylococcus aureus with MICs of 4 and 2μg·mL^(−1),respectively,which also exhibited DPPH radical scavenging activity comparable to the positive control with IC50 values of 9.2±0.03 and 13.3±0.01μmol·L^(−1),respectively.