A betaxanthin-based visible and fluorescent reporter for monitoring plant transformation

作     者：Yuanjie Deng Aoqi Duan Tong Li Huiru Wang Aisheng Xiong Yuanjie Deng;Aoqi Duan;Tong Li;Huiru Wang;Aisheng Xiong

作者机构：State Key Laboratory of Crop Genetics and Germplasm EnhancementMinistry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural UniversityNanjing 210095JiangsuChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2023年第11卷第2期

页      面：666-671页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Natural Science Foundation of China(31872098,32072563) Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD) 

主　　题：Reporter Betaxanthins Visible Fluorescent Plant transformation 

摘      要：Commonly used reporters rely on a single property,such as the fluorescence of GFP and visible color of anthocyanins,therefore these reporters hardly handle the complicated condition in *** are a group of plant natural products derived from the amino acid *** visible yellow-orange color and green fluorescence under blue light make it a promising new *** two enzymatic reactions are required to convert tyrosine into ***,we synthesized an open reading frame named Bx that contained all the betaxanthins biosynthetic genes and demonstrated its use as a powerful and efficient reporter in tobacco,carrot,and tomato.