Subcellular Localization Analysis of Bovine Foamy Virus Borf1 Protein

作     者：Juan TAN Kai WU Rui CHANG Qi-min CHEN Yun-qi GENG Wen-tao QIAO 

作者机构：The Key Laboratory of Molecular Microbiology and Techaology Ministry of Education and Tianjin Key Laboratory of Microbial Functional Genomics College of Life Sciences Nankai University Tianjin 300071 China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2008年第23卷第1期

页      面：37-42页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：National Natural Science Foundation ofChina (30570072, 30770097) Natural Science Foun-dation of Tianjin (05YFJM- JC01000) 

主　　题：Bovine foamy virus (BFV) Borfl Antiserum 

摘      要：The Borfl protein is encoded by an immediate-early gene of the bovine foamy virus （BFV） and plays a key role in the viral life cycle. Borfl is a DNA binding protein which can transactivate both the long terminal repeat （LTR） and the internal promoter （IP） of BFV by specifically binding to the transactivation responsive element （TRE）. To analyze the subcellular localization of Borfl during the BFV life cycle, this gene was cloned into a prokaryotic expression vector and expressed in a soluble form. After the purification and immunization, we raised the mouse anti-Borfl serum with a high titer based on ELISA results. Western blot analysis showed that the antiserum could specifically recognize the Borfl protein that was expressed in 293T cells. With this specific serum, we revealed the nuclear and cytoplasmic localization of Borfl in HeLa cells that was transfected with Borfl. Moreover, the immuno-fluorescence assay also showed that the localization of Borfl during the infection and transfection of BFV was identical.