Determination of L-ergothioneine in food by UPLC-MS/MS method
作者机构：Hebei Food Safety Key LaboratoryKey Laboratory of Special Food Supervision Technology for State Market RegulationHebei Engineering Research Center for Special Food Safety and HealthHebei Food Inspection and Research InstituteShijiazhuang 050227China
出 版 物：《Journal of Future Foods》 (未来食品学报（英文）)
年 卷 期：2023年第3卷第2期
页 面：163-168页
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学]
基 金：This work was supported by National Key Research and Development Program of China(2019YFC1606400)
主 题：L-Ergothioneine Ultra-performance liquid chromatography tandem mass spectrometry Hydrophilic interaction liquid chromatography Food Determination
摘 要：L-Ergothioneine(L-EGT)possesses excellent antioxidant activity and has been used in the food,pharmaceuticals and cosmetics *** this study,a new efficient and sensitive ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method was established for the quantitative determination of L-EGT in *** sample was extracted with methanol-water(70:30,V/V),separated by hydrophilic interaction liquid chromatography(HILIC)and detected by triple-quadrupole mass *** studies were carried out on different product and the limit of quantitation was 20μg/kg(milk,alcohol-free beverages,dairy products)and 40µg/kg(cereal bars,chocolate).Excellent linearity(correlation coefficient(R2)≥0.999)was achieved for L-EGT quantification in the range of 5–200 ng/*** recoveries of the method(83.7%−107.5%)and the relative standard deviation(RSD,0.88%−6.84%(n=6))meet the performance criteria required for the determination of L-EGT in ***,the applicability of the method was tested by analysing actual *** general,the method developed is simple,reliable,accurate,and stable and could be useful for routine analyses of L-EGT in food.
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