The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice

作     者：Xiaowei Fan Pengfei Wang Feixiang Qi Yong Hu Shuangle Li Jia Zhang Liwen Liang Zhanyi Zhang Juhong Liu Lizhong Xiong Yongzhong Xing Xiaowei Fan;Pengfei Wang;Feixiang Qi;Yong Hu;Shuangle Li;Jia Zhang;Liwen Liang;Zhanyi Zhang;Juhong Liu;Lizhong Xiong;Yongzhong Xing

作者机构：National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanHubei 430070China Hubei Hongshan LaboratoryWuhanHubei 430070China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2023年第50卷第10期

页      面：755-764页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the Natural Science Foundation of China(U20A2031) the Earmarked Fund for China Agricultural Research System(CARS-01) 

主　　题：Heading date CCT family gene ChIP-seq Constant repressor Rice(Oryza sativa L.) 

摘      要：CONSTANS,CO-like,and TOC1(CCT)family genes play important roles in regulating heading date,which exerts a large impact on the regional and seasonal adaptation of *** studies have shown that Grain number,plant height,and heading date2(Ghd2)exhibits a negative response to drought stress by directly upregulating Rubisco activase and exerting a negative effect on heading ***,the target gene of Ghd2 regulating heading date is still *** this study,CO3 is identified by analyzing Ghd2 ChIP-seq ***2 activates CO3 expression by binding to the CO3 promoter through its CCT *** experiments show that the motif CCACTA in the CO3 promoter was recognized by Ghd2.A comparison of the heading dates among plants with CO3 knocked out or overexpressed and double-mutants with Ghd2 overexpressed and CO3 knocked out shows that CO3 negatively and constantly regulates flowering by repressing the transcription of Ehd1,Hd3a,and *** addition,the target genes of CO3 are explored via a comprehensive analysis of DAP-seq and RNA-seq *** together,these results suggest that Ghd2 directly binds to the downstream gene CO3,and the Ghd2eCO3 module constantly delays heading date via the Ehd1-mediated pathway.