The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice
作者机构:National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanHubei 430070China Hubei Hongshan LaboratoryWuhanHubei 430070China
出 版 物:《Journal of Genetics and Genomics》 (遗传学报(英文版))
年 卷 期:2023年第50卷第10期
页 面:755-764页
核心收录:
基 金:supported by the Natural Science Foundation of China(U20A2031) the Earmarked Fund for China Agricultural Research System(CARS-01)
主 题:Heading date CCT family gene ChIP-seq Constant repressor Rice(Oryza sativa L.)
摘 要:CONSTANS,CO-like,and TOC1(CCT)family genes play important roles in regulating heading date,which exerts a large impact on the regional and seasonal adaptation of *** studies have shown that Grain number,plant height,and heading date2(Ghd2)exhibits a negative response to drought stress by directly upregulating Rubisco activase and exerting a negative effect on heading ***,the target gene of Ghd2 regulating heading date is still *** this study,CO3 is identified by analyzing Ghd2 ChIP-seq ***2 activates CO3 expression by binding to the CO3 promoter through its CCT *** experiments show that the motif CCACTA in the CO3 promoter was recognized by Ghd2.A comparison of the heading dates among plants with CO3 knocked out or overexpressed and double-mutants with Ghd2 overexpressed and CO3 knocked out shows that CO3 negatively and constantly regulates flowering by repressing the transcription of Ehd1,Hd3a,and *** addition,the target genes of CO3 are explored via a comprehensive analysis of DAP-seq and RNA-seq *** together,these results suggest that Ghd2 directly binds to the downstream gene CO3,and the Ghd2eCO3 module constantly delays heading date via the Ehd1-mediated pathway.