Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection

作     者：Jiale Gao Xiaodong Sun Yongxin Liu Bing Niu Qin Chen Xueen Fang Jiale Gao;Xiaodong Sun;Yongxin Liu;Bing Niu;Qin Chen;Xueen Fang

作者机构：Shanghai Key Laboratory of Bio-Energy CropsSchool of Life SciencesShanghai UniversityShanghai 200444China Shanghai Engineering Research Center of Organ RepairSchool of MedicineShanghai UniversityShanghai 200444China Department of Chemistry and Institutes of Biomedical SciencesFudan UniversityShanghai 200433China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2023年第12卷第5期

页      面：1788-1798页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 09[农学] 0903[农学-农业资源与环境] 

基　　金：the Science and Technology Joint Project of the Yangtze River Delta (19395810100) Shanghai Agriculture Project (19391901500) the National Key Research and Development Program of China (2016YFD0501101) the Shanghai Science and Technology Innovation Foundation (19441903900 and 19XD1433000) Project of National Natural Science Foundation of China (82003705) 

主　　题：Cu-TCPP nanosheet Plant allergen Nucleic acid detection Loop-mediated isothermal amplification 

摘      要：The rapid and accurate detection of peanuts and soybeans allergen is important to the food safety. In this study, Cu-TCPP nanosheet, a kind of ultra-thin metal-organic framework(MOF)was synthesized and applied in loop-mediated isothermal amplification(named Cu-TCPP@LAMP), which can inhibit the non-specific amplification by absorbing and precise temperature releasing of single primer. As thus, Cu-TCPP@LAMP can achieve high sensitivity and specific amplification of the target gene. As a result, peanut and soybean allergens genes contained in food were successfully detected with a favorable detection sensitivity(5 ng/μL for peanuts and 10 ng/μL for soybeans)and reliable repeatability(The coefficient of variation was 3.38% for peanuts and 3.33% for soybeans). Moreover, the established method was utilized for detection of several commercial products, and had a high consistency with the standard method. Apart from food allergens, this novel assay can be widely used in other areas, such as pathogen detection, tumor nucleic acid detection and so on.