Near infrared light-induced dynamic modulation of enzymatic activity through polyphenol-functionalized liquid metal nanodroplets

作     者：Chaojun Zhang Yang Tang Qin Wang Yunxiang He Xiaoling Wang Sebastian Beyer Junling Guo Chaojun Zhang;Yang Tang;Qin Wang;Yunxiang He;Xiaoling Wang;Sebastian Beyer;Junling Guo

作者机构：BMI Center for Biomass Materials and NanointerfacesCollege of Biomass Science and EngineeringSichuan UniversityChengdu 610065China School of PharmacySouthwest Minzu UniversityChengdu 610225China National Engineering Research Center of Clean Technology in Leather IndustrySichuan UniversityChengdu 610065China Department of Biomedical EngineeringChinese University of Hong KongHong Kong 999077China State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065China Bioproducts InstituteDepartment of Chemical and Biological EngineeringUniversity of British ColumbiaVancouverBC V6T 1Z4Canada 

出 版 物：《中国化学快报：英文版》 (Chinese Chemical Letters)

年 卷 期：2023年第34卷第2期

页      面：475-480页

核心收录：

学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：financial support from the National Talents Program, National Natural Science Foundation of China (Nos. 22178233, 22108181) Talents Program of Sichuan Province, Double First-Class University Plan of Sichuan University, State Key Laboratory of Polymer Materials Engineering (No. sklpme 2020-03-01) the Sichuan Province Postdoctoral Special Funding 

主　　题：Enzymatic activity Photo-modulation Near infrared light Liquid metal nanodroplets Metal-phenolic nanocoating 

摘      要：Dynamic manipulation of enzymatic activity is a challenging task for applications in chemical and pharmaceutical industries due to the difficult modification and variable conformation of various ***, we report a new strategy for reversible dynamic modulation of enzymatic activity by near-infrared light-induced photothermal conversion based on polyphenol-functionalized liquid metal nanodroplets(LM). The metal-phenolic nanocoating not only provides colloidal stability of LM nanodroplets but also generates nanointerfaces for the assembly of various enzymes on the LM nanodroplets. Upon near infrared(NIR) irradiation, the localized microenvironmental heating through photothermal effect of the LM nanodroplets allows tailoring the enzymatic activity without affecting the bulk temperature. A library of functional enzymes, including proteinase K, glucoamylase, glucose oxidase, and Bst DNA polymerase, is integrated to perform a reversible control and enhanced activities even after five times of cycles, demonstrating great potential in bacterial fermentation, bacteriostasis, and target gene amplification.