circRNAs deregulation in exosomes derived from BEAS-2B cells is associated with vascular stiffness induced by PM_(2.5)

作     者：Qingping Liu Yaling Zhang Bin Han Mengruo Wang Huaifang Hu Jie Ning Wentao Hu Meiyu Chen Yaxian Pang Yuanyuan Chen Lei Bao Yujie Niu Rong Zhang Qingping Liu;Yaling Zhang;Bin Han;Mengruo Wang;Huaifang Hu;Jie Ning;Wentao Hu;Meiyu Chen;Yaxian Pang;Yuanyuan Chen;Lei Bao;Yujie Niu;Rong Zhang

作者机构：Department of ToxicologyHebei Medical UniversityShijiazhuang 050017China State Key Laboratory of Environment Health(Incubation)Key Laboratory of Environment and HealthMinistry of EducationKey Laboratory of Environment and Health(Wuhan)Ministry of Environmental ProtectionSchool of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China China CDC Key Laboratory of Environment and Population HealthNational Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China Occupational Health and Environmental HealthHebei Medical UniversityShijiazhuang 050017China Hebei Key Laboratory of Environment and Human HealthHebei Medical UniversityShijiazhuang 050017China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2024年第137卷第3期

页      面：527-539页

核心收录：

学科分类：100405[医学-卫生毒理学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 07[理学] 070602[理学-大气物理学与大气环境] 0706[理学-大气科学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (Nos. 92043202, 91643108, and 81973074) Foundation by Science and Technology Project of Hebei Education Department (No. QN2020175) 

主　　题：Fine particulate matter Vascular stiffness Exosome ceRNA microarray Endothelial dysfunction 

摘      要：As an environmental pollutant,ambient fine particulate matter (PM_(2.5)) was linked to cardiovascular *** molecular mechanisms underlying PM_(2.5)-induced extrapulmonary disease has not been elucidated *** this study the ambient PM_(2.5)exposure mice model we established was to explore adverse effects of vessel and potential ***-term PM_(2.5)exposure caused reduced lung function and vascular stiffness in *** chronic PM_(2.5)induced migration and epithelial-mesenchymal transition (EMT) phenotype in BEAS-2B *** PM_(2.5)treatment,the circRNAs and mRNAs levels of exosomes released by BEAS-2B cells were detected by competing endogenous RNA (ceRNA) array,which contained 1664 differentially expressed circRNAs (DE-circRNAs) and 308 differentially expressed m RNAs (DE-mRNAs).By bioinformatics analysis on host genes of DE-circRNAs,vascular diseases and some pathways related to vascular diseases including focal adhesion,tight junction and adherens junction were ***,ceRNA network was constructed,and DE-mRNAs in ceRNA network were conducted functional enrichment analysis by Ingenuity Pathway Analysis,which indicated that hsa_circ_0012627,hsa_circ_0053261and hsa_circ_0052810 were related to vascular endothelial ***,it was verified experimentally that Exo^(PM2.5)could induce endothelial dysfunction by increased endothelial permeability and decreased relaxation in *** present study,we investigated in-depth knowledge into the molecule events related to PM_(2.5) toxicity and pathogenesis of vascular diseases.