Methionine oxidation and reduction of the ethylene signaling component MaEIL9 are involved in banana fruit ripening

作     者：Lisha Zhu Lin Chen Chaojie Wu Wei Shan Danling Cai Zengxiang Lin Wei Wei Jianye Chen Wangjin Lu Jianfei Kuang Lisha Zhu;Lin Chen;Chaojie Wu;Wei Shan;Danling Cai;Zengxiang Lin;Wei Wei;Jianye Chen;Wangjin Lu;Jianfei Kuang

作者机构：State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products PreservationMinistry of EducationCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China)Ministry of Agriculture and Rural AffairsCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第1期

页      面：150-166页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：funded by the National Natural Science Foundation of China (31972116) Guangdong Special Support Program (2017TQ04N512) China Agriculture Research System of Ministry of Finance (MOF)and Ministry of Agriculture and Rural Affairs (MARA) (CARS-31) Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams (2022KJ109)。 

主　　题：banana EIN3/EIL redox regulation ROS starch degradation 

摘      要：The ethylene insensitive 3/ethylene insensitive3-like(EIN3/EIL)plays an indispensable role in fruit ripening.However,the regulatory mechanism that links post-translational modification of EIN3/EIL to fruit ripening is largely unknown.Here,we studied the expression of 13 MaE IL genes during banana fruit ripening,among which MaE IL9 displayed higher enhancement particularly in the ripening stage.Consistent with its transcript pattern,abundance of MaE IL9 protein gradually increased during the ripening process,with maximal enhancement in the ripening.DNA affinity purification(DAP)-seq analysis revealed that MaE IL9 directly targets a subset of genes related to fruit ripening,such as the starch hydrolytic genes MaA MY3D and MaB AM1.Stably overexpressing MaE IL9 in tomato fruit hastened fruit ripening,whereas transiently silencing this gene in banana fruit retarded the ripening process,supporting a positive role of MaEIL9 in fruit ripening.Moreover,oxidation of methionines(Met-129,Met-130,and Met-282)in MaEIL9 resulted in the loss of its DNA-binding capacity and transcriptional activation activity.Importantly,we identified MaEIL9 as a potential substrate protein of methionine sulfoxide reductase A MaMsrA4,and oxidation of Met-129,Met-130,and Met-282in MaEIL9 could be restored by MaMsrA4.Collectively,our findings reveal a novel regulatory network controlling banana fruit ripening,which involves MaMsrA4-mediated redox regulation of the ethylene signaling component MaEIL9.