Temporal transcriptome reveals that circadian clock is invoived in the dynamic regulation of immune response to bacterial infection in Bombyx mori

作     者：Jian-Feng Qiu Wen-Zhao Cui Qiang Zhang Tai-Ming Dai Kai Liu Jiang-Lan Li Yu-Jun Wang Yang-Hu Sima Shi-Qing Xu 

作者机构：School of Biology and Basic Medical SciencesSuzhou Medical CollegeSoochow UniversitySuzhouChinaJiangsu Province Institute of Agricultural Biotechnology&Ecology(IABE)Soochow UniversitySuzhouChinaJiangsu Province Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity ConservationCollege of Marine SciencesBeibu Gulf UniversityQinzhouChinaGuangxi Province 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2023年第30卷第1期

页      面：31-46页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(Grant No.32172794 to S.-Q.X.and 31760713 to Y.-J.W.) China Postdoctoral Science Foundation(Grant No.2021M692343 to J.-F.Q.) Nantong Science and Technology(Grant No.JC2021073 to J.-F.Q.) the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD to S.-Q.X.) the China Agriculture Research System of MOF and MARA(Grant No.CARS-18-ZJ0103 to Y.-H.S.) 

主　　题：Bombyx mori circadian clock cry 1 knockout immune response 

摘      要：The circadian clock plays a critical role in the regulation of host immune ***,the mechanistic basis for this regulation is largely ***,the core clock gene cryptochromel(cryl)knockout line in Bombyx mori,an invertebrate animal model,was constructed to obtain the silkworm with dysfunctional molecular clock,and the dynamic regulation of the circadian clock on the immune responsiveness within 24h of Staphylococcus aureus infection was *** found that deletion of cryl decreased viability of silkworms and significantly reduced resistance of larvae to *** series RNA-seq analysis identified thousands of rhythmically expressed genes,including immune response genes,in the larval immune tissue,fat *** cry1 knockout silkworms exhibited expression patterns of rhythmically expressed genes similar to wild-type(WT)silkworms infected with ***,cry knockout silkworms exhibited a seriously weakened response to *** *** immune response peaked at 6 and 24 h after infection,during which“transcription stormsoccurred,and the expression levels of the immune response genes,PGRP and antimicrobial peptides(AMPs),were significantly upregulated in *** contrast,cry I knockout did not effectively activate Toll,Imd,or NF-κB signaling pathways during the immune adjustment period from 12 to 18 h after infection,resulting in failure to initiate the immune responsiveness peak at 24 h after *** may be related to inhibited silkworm fat body energy *** results demonstrated the dynamic regulation of circadian clock on silkworm immune response to bacterial infection and provided important insights into hostantimicrobial defensemechanisms.