Mobile ARGONAUTE 1d binds 22-nt miRNAs to generate phasiRNAs important for low-temperature male fertility in rice

作     者：Fuyan Si Haofei Luo Chao Yang Jie Gong Bin Yan Chunyan Liu Xianwei Song Xiaofeng Cao 

作者机构：State Key Laboratory of Plant Genomics and National Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China The Municipal Key Laboratory of the Molecular Genetics of Hybrid WheatInstitute of Hybrid WheatBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China Innovative Academy of Seed Design(INASEED)Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China College of Life SciencesUniversity of the Chinese Academy of SciencesBeijing 100039China CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesBeijing 100101China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2023年第66卷第2期

页      面：197-208页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31788103,32170620) the Chinese Academy of Sciences(QYZDY-SSW-SMC022,XDB27030201,XDA24010302) the State Key Laboratory of Plant Genomics。 

主　　题：mobile signal ARGONAUTE 22-nt miRNA phasiRNA low temperature male sterility rice 

摘      要：Phased small interfering RNAs(phasiRNAs) are abundantly expressed in anthers and linked to environment-related male fertility in grasses, yet how they function under different environmental conditions remains unclear. Here, we identified a rice(Oryza sativa) low temperature-induced Argonaute(AGO) protein, OsAGO1d, that is responsible for generating phasiRNAs and preserving male fertility at low temperature. Loss of OsAGO1d function causes low-temperature male sterility associated with delayed programmed cell death of tapetal cells during anther development. OsAGO1d binds miR2118 and miR2275 family members and triggers phasiRNA biogenesis;it also binds 21-nt phasiRNAs with a 5′ terminal U. In total, phasiRNAs from 972loci are OsAGO1d-dependent. OsAGO1d protein moves from anther wall cells into meiocytes, where it loads miR2275 to produce 24-nt phasiRNAs. Together, our results show that OsAGO1d acts as a mobile signal to fine-tune phasiRNA production and this function is important for male fertility at low temperature.