Identification of anti-inflammatory components in Panax ginseng of Sijunzi Decoction based on spectrum-effect relationship

作     者：Hong Kan Dongxue Zhang Weijia Chen Shihan Wang Zhongmei He Shifeng Pang Shuai Qu Yingping Wang Hong Kan;Dongxue Zhang;Weijia Chen;Shihan Wang;Zhongmei He;Shifeng Pang;Shuai Qu;Yingping Wang

作者机构：College of Chinese Medicinal MaterialsJilin Agricultural UniversityChangchun 130118China Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural SciencesChangchun 130112China Jilin Institute of BiologyChangchun 130012China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2023年第15卷第1期

页      面：123-131页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：financially supported by Science Foundation of Jilin Educational Committee (No. JJKH20200363KJ) Jilin Science & Technology Development Plan (No. 20190304009YY). Jilin Science & Technology Development Plan (No. 20200404090YY) 

主　　题：anti-inflammation ginsenosides Panax ginseng C.A.Mey Sijunzi Decoction spectrum-effect relationship ulcerative colitis 

摘      要：Objective: This study aimed to identify the main medicinal active components of Panax ginseng(P. ginseng) in the compatibility environment of clinical application. For this purpose, the anti-inflammatory ingredients of P. ginseng were investigated based on its therapeutic effect in Sijunzi Decoction(SJD) which is a widely used traditional Chinese ***: The fingerprints of 10 batches of SJD consisting of different sources of P. ginseng were established by UPLC technique to investigate the chemical components. At the same time, the antiinflammatory effects of these components were evaluated by dextran sulfate sodium-induced ulcerative colitis mouse model. Grey relational analysis was applied to explore the correlation degree between fingerprints and anti-inflammatory effects in SJD. Lipopolysaccharide-stimulated RAW264.7 murine macrophages were established to evaluate the anti-inflammatory action of the screened effective substances of P. ***: According to grey relational analysis, notoginsenoside R1, ginsenoside Rg2 and ginsenoside Rb3of P. ginseng were the major anti-inflammatory contributions in SJD. They had been proven to be closely associated with the anti-inflammatory process of SJD and displayed a close effect compared with SJD by LPS-stimulated RAW264.7 murine ***: Our work provides a general strategy for exploring the pharmacological ingredients of P. ginseng in traditional Chinese formulas which is beneficial for establishing the quality standards of traditional herbs in traditional Chinese medicine prescription based on their clinical therapeutic effect.