Colorectal cancer-derived extracellular vesicles induce liver premetastatic immunosuppressive niche formation to promote tumor early liver metastasis

作     者：Xuyang Yang Yaguang Zhang Yang Zhang Huifang Li Li Li Yangping Wu Xiangzheng Chen Lei Qiu Junhong Han Ziqiang Wang Xuyang Yang;Yaguang Zhang;Yang Zhang;Huifang Li;Li Li;Yangping Wu;Xianqzheng Chen;Lei Qiu;Junhong Han;Ziqiang Wang

作者机构：Colorectal Cancer CenterDepartment of General SurgeryState Key Laboratory of Biotherapy and Cancer Centerand Frontiers Science Center for Disease-related Molecular NetworkWest China HospitalSichuan UniversityChengdu 610041 Sichuan ProvinceChina State Key Laboratory of Biotherapy and Cancer Centerand Frontiers Science Center for Disease-related Molecular NetworkWest China HospitalSichuan UniversityChengdu 610041 Sichuan ProvinceChina Core FacilityInstitute of Clinical PathologyDepartment of Respiratory and Critical Care Medicineand Department of Liver Surgery&Liver TransplantationState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041China 

出 版 物：《Signal Transduction and Targeted Therapy》 (信号转导与靶向治疗（英文）)

年 卷 期：2023年第8卷第4期

页      面：1357-1360页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the Department of Science and Technology of Sichuan Province(2019YFS0375 2021YFS0025 2022YFS0162) 1·3·5 project for disciplines of excellence,West China Hospital,Sichuan University(20HXJS003 ZYGD18003) 1·3·5 project for disciplines of excellence-Clinical Research Incubation Project,West China Hospital,Sichuan University(22HXFH001,2019HXFH031) a Post-Doctoral Research Project,West China Hospital,Sichuan University(2021HXBH033) a Post-Doctoral Research Project,Sichuan University(20826041E4084) the China Postdoctoral Science Foundation(2022M712264) the National Natural Science Foundation of China(82103541) 

主　　题：metastasis metastatic cancer 

摘      要：Dear Editor,To date,the effect of colorectal cancer(CRC)-derived extracellular vesicles(EVs)on liver pre-metastatic niche(PMN)remain incompletely understood.1 To investigate the role of CRC-derived EVs in the remodeling of the liver PMN,we isolated EVs from CT26 cell culture *** characteristics of EVs(the morphology,size,and markers)were identified by transmission electron microscopy(TEM),Nanoparticle Tracking Analysis(NTA),and western blotting(Supplementary Fig.S1a–e).Then,BALB/c mice with an intact liver immune status were pretreated with CRCderived EVs and phosphate-buffered saline(PBS)for one month,and a liver metastasis model was established via spleen injection of tumor cells to determine whether EVs influence liver metastasis(Fig.1a).Two hours after the operation,in vivo imaging indicated that the animal model was successfully established,and the tumor fluorescence intensity in the liver was consistent between the two groups(Supplementary Fig.S2a,b).Amazingly,the liver tumor fluorescence intensity in the EVs group was significantly stronger than that in the PBS group after 24 h(Fig.1b;Supplementary Fig.S2c,d).The number of liver tumor nodules in the EVs group was also significantly higher than that in the PBS group on day 4(Fig.1c;Supplementary Fig.S2e,f).