Identification and Functional Analysis of Serum Specific miRNAs in Recurrent Aphthous Stomatitis Patients with Excess-heat or Yin-deficiency

作     者：Jie Bao Zhengyang Zhu Xizhao Zhang Lin Huang Li Xu Xiaobing Dou Yongsheng Fan 

作者机构：School of Basic Medical SciencesZhejiang Chinese Medical UniversityHangzhou 310053ZhejiangChina Cancer Hospital of The University of Chinese Academy of SciencesHangzhou 310005ZhejiangChina The First School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhou 310053ZhejiangChina School of Life SciencesZhejiang Chinese Medical UniversityHangzhou 310053ZhejiangChina 

出 版 物：《Clinical Complementary Medicine and Pharmacology》 (临床补充医学和药理学（英文）)

年 卷 期：2023年第3卷第4期

页      面：10-18页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by National Basic Research Program of China(973 Program)(Grant No.2014CB543000) the National Natural Science Foundation of China(Grant No.81803980) the National Natural Science Foundation of Zhejiang Province(Grant No.LQ18H270004) 

主　　题：Recurrent aphthous stomatitis MicroRNA Excess-heat syndrome Yin-deficiency syndrome 

摘      要：Background:The treatment of Traditional Chinese Medicine(TCM)in recurrent aphthous stomatitis(RAS)based on syndrome differentiation has won the international acceptance,but the molecular mechanism of excess-heat syndrome and yin-deficiency syndrome of RAS remains ***:To clarify specific microRNAs(miRNAs)and their functions in RAS patients with excess-heat or ***:Serum samples were collected from patients meeting the RAS diagnostic criteria of excess-heat or yin-deficiency syndrome and healthy *** miRNAs were then identified under miRNA microarray *** prediction and bioinformatic analyses were carried out and gene-pathway-networks were visualized to better understand the relationship between different genes and ***:(1)90 individuals meeting the inclusion criteria were collected in this *** them,9 miRNAs were screened out in excess-heat syndrome group(EH group),with 1 upregulated and 8 *** four miRNAs(hsa-miR-20b-5p,hsa-miR-122-5p,hsa-miR-483-5p and hsa-miR-3197)were validated by real-time PCR method.14 miRNAs were screened out in yin-deficiency syndrome group(YD group)(7 upregulated and 7 downregulated).And hsa-miR-17-5p,hsa-miR-106-5p and hsa-miR-20b-5p were validated.(2)A total of 4,776 target genes were identified in EH group which enriched in GO categories including nervous system development and calcium ion binding and pathway such as PI3K-Akt signaling pathway and Calcium signaling pathway.10,172 target genes were identified in YD group which enriched GO categories included protein binding and positive regulation of transcription from RNA polymerase II promoter and pathway included MAPK signaling pathway and Ras signaling ***:Hsa-miR-20b-5p in patients with RAS could act as the novel target for the classification of the *** is upregulated in RAS patients with excess-heat syndrome while downregulated in patients with yin-deficiency *** PI