Dysregulated RUNX1 Predicts Poor Prognosis by Mediating Epithelialmesenchymal Transition in Cervical Cancer

作     者：Ling-ling ZHENG Lei CAI Xiao-qing ZHANG Zhe LEI Chang-sheng YI Xing-dang LIU Ji-gang YANG Ling-ling ZHENG;Lei CAI;Xiao-qing ZHANG;Zhe LEI;Chang-sheng YI;Xing-dang LIU;Ji-gang YANG

作者机构：Department of Nuclear MedicineBeijing Friendship HospitalCapital Medical UniversityBeijing100050China Department of Nuclear MedicineHuashan HospitalFudan UniversityShanghai200040China Reproductive Medicine CenterTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Thoracic Surgerythe Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhou450003China 

出 版 物：《Current Medical Science》 (当代医学科学（英文）)

年 卷 期：2022年第42卷第6期

页      面：1285-1296页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 10[医学] 

主　　题：runt-related transcription factor 1 cervical cancer epithelial-mesenchymal transition bioinformatics 

摘      要：Objective Runt-related transcription factor 1(RUNX1)has been proven to be over-expressed and vital in many malignancies.However,its role in cervical cancer is still unclear.Methods Some online databases(Oncomine,GEPIA,UALCAN,LinkedOmics,and others)were used to explore the expression level,prognostic significance,and gene mutation characteristics of RUNX1 in cervical cancer.The protein levels of RUNX1 in cervical cancer were measured by immunohistochemistry(IHC).The functional changes of cervical cancer cells were measured in vitro after decreasing RUNX1.Results Bioinformatic results revealed that RUNX1 was upregulated in cervical cancer compared to normal tissues.Moreover,over-expression of RUNX1 was significantly correlated with cervical cancer patients’clinical parameters(e.g.,individual cancer stages,patients’age,nodal metastasis status,and others).Meanwhile,functional enrichment analysis of RUNX1-related genes indicated that RUNX1 was mainly involved in the epithelial-mesenchymal transition(EMT)process in cervical cancer.Furthermore,RUNX1 may be upregulated by hsamiR-616-5p and hsa-miR-766 identified by miRDB,TargetScan,and miRWalk.Finally,RUNX1 was upregulated in cervical cancer compared to normal tissues by IHC in collected cervical cancer samples.The invasion and migration abilities of cervical cancer cells were significantly reduced by repressing EMT after knocking down RUNX1 in vitro.Conclusion RUNX1 was highly expressed in cervical cancer,and upregulated RUNX1 could significantly promote the invasive abilities of cervical cancer cells by inducing EMT.Therefore,RUNX1 may be a potential biomarker for early diagnosis and targeted therapy of cervical cancer.