Network pharmacology and verification experiment-based prediction of active components and potential targets of Alpiniae Oxyphyllae Fructus-Saposhnikoviae Radix(Yizhiren-Fangfeng)for treatment of diabetic kidney disease
作者机构：School of Traditional Chinese MedicineHainan Medical UniversityHaikou 571199China School of Basic Medicine and the Life SciencesHainan Medical UniversityHaikou 571199China
出 版 物：《Traditional Medicine Research》 (TMR传统医学研究)
年 卷 期：2023年第8卷第5期
页 面：11-20页
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：the National Natural Science Foundation of China(grant No.82160897,82205087) Hainan Provincial Natural Science Foundation of China(grant No.820RC635) Scientific Research Foundation of Hainan Medical University(grant No.HYPY201924,HYPY2020037) Hainan Medical University 2020 National Innovation and Entrepreneurship Program for College Students(grant No.202011810006)
主 题：network pharmacology traditional Chinese medicine Alpiniae Oxyphyllae Fructus Saposhnikoviae Radix HK-2 cells diabetic kidney disease
摘 要：Background:In this study,we analyzed the potential active components,related crucial targets and possible signaling pathway mechanisms of Alpiniae Oxyphyllae Fructus and Saposhnikoviae Radix(AOF-SR)herb pairs in the treatment of diabetic kidney disease(DKD)using network pharmacology and verification ***:The active compounds and potential targets of AOF-SR were derived from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,The Encyclopedia of Traditional Chinese Medicine,and PubChem databases,and the potential therapeutic targets of DKD were derived from the OMIM,Drugbank,and DisGeNET ***“compounds-diseases-targetsnetwork was constructed using Cytoscape *** functionality in Cytoscape is being used to screen important targets for AOF-SR treatment of *** Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of important targets were performed using DAVID *** addition,according to the predicted results of network pharmacology,HK-2 cells were used to construct DKD model for verification ***-2 cells were divided into control group,high glucose(HG)group and AOF-SR(HG+AOF-SR)group to detect survival rate and expression of key proteins in NF-κB and PI3K/Akt signaling ***:A total of 38 compounds were selected from AOF-SR,of which 23 were Alpiniae Oxyphyllae Fructus and 15 were Saposhnikoviae *** enrichment analysis of 82 important targets,88 signaling pathways were identified;some of these pathways,such as the NF-κB,PI3K-Akt,IL-17,and JAK/STAT signaling pathways,regulate the pathological process of *** verification experiment,the HK-2 cells survival rate was higher in the HG+AOF-SR group than in the HG group(P0.05).Moreover,western blotting results showed that the expression levels of NF-κB,p-PI3K,and p-Akt in HG+AOF-SR group were significantly lower than those in HG group(P0.05).Conclusion:Overall,this study revealed the active co
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