Label-free imaging immune cells and collagen in atherosclerosis with two-photon and second harmonic generation microscopy

作     者：Chunqiang Li Riikka K.Pastila Charles P.Lin 

作者机构：Department of Physics The University of Texas at El Paso El PasoTexas 79968USA Wellman Center for Photomedicine and Center for Systems Biology Massachusetts General Hospital Harvard Medical School 185 Cambridge St.Boston Massachusetts 02114USA TUK-Radiation and Nuclear Safety Authority Laippatie 4FI-00880 HelsinkiFinland 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2016年第9卷第1期

页      面：39-46页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0836[工学-生物工程] 10[医学] 0702[理学-物理学] 

基　　金：This study was supported by United States NIH P41 EB015903-02S1 grant awarded to CPL. 

主　　题：Two-photon microscopy mast cells atherosclerosis 

摘      要：Atherosclerosis has been recognized as a chronic inflammation disease,in which many types of cells participate in this process,including lymphocytes,macrophages,dendritic cells(DCs),mast cells,vascular smooth muscle cells(SMCs).Developments in imaging technology provide the capability to observe cellular and tissue components and their interactions.The knowledge of the functions of immune cells and their interactions with other cell and tissue components will facilitate our discovery of biomarkers in atherosclerosis and prediction of the risk factor of rupture-prone plaques.Nonlinear optical microscopy based on two-photon excited autofluorescence and second harmonic generation(SHG)were developed to image mast cells,SMCs and collagen in plaque ex vivo using endogenous optical signals.Mast cells were imaged with two-photon tryptophan autofluorescence,SMCs were imaged with two-photon NADH auto fluorescence,and collagen were imaged with SHG.This development paves the way for further study of mast cell degranulation,and the effects of mast cell derived mediators such as induced synthesis and activation of matrix metalloproteinases(MMPs)which participate in the degradation of collagen.