Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway

作     者：Ye Eun Joo Pil-Soo Jeong Sanghoon Lee Se-Been Jeon Min-Ah Gwon Min Ju Kim Hyo-Gu Kang Bong-Seok Song Sun-Uk Kim Seong-Keun Cho Bo-Woong Sim Ye Eun Joo;Pil-Soo Jeong;Sanghoon Leee;Se-Been Jeon;Min-Ah Gwon;Min Ju Kim;Hyo-Gu Kang;Bong-Seok Song;Sun-Uk Kim;Seong-Keun Cho;Bo-Woong Sim

作者机构：Futuristic Animal Resource and Research CenterKorea Research Institute of Bioscience and BiotechnologyCheongjuSouth Korea Department of Animal ScienceCollege of Natural Resources and Life SciencePusan National UniversityMiryangSouth Korea Laboratory of TheriogenologyCollege of Veterinary MedicineChungnam National UniversityDaejeonSouth Korea Department of BiotechnologyCollege of EngineeringDaegu UniversityGyeongsanSouth Korea Department of Animal Science and BiotechnologyCollege of Agriculture and Life ScienceChungnam National UniversityDaejeonSouth Korea Department of Functional GenomicsUniversity of Science and TechnologyDaejeonSouth Korea Department of Animal ScienceCollege of Natural Resources and Life ScienceLife and Industry Convergence Research InstitutePusan National UniversityMiryangSouth Korea 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2023年第14卷第4期

页      面：1395-1407页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：supported by the Ministry of Education Science and Technology(No.2021M3A9A1096894) Republic of Korea and the KRIBB Research Initiative Program(KGM4252223) Korea Research Institute of Bioscience and Biotechnology,Republic of Korea 

主　　题：Anethole Lipid metabolism Mitochondrial function Porcine embryo development Sonic hedgehog signaling pathway 

摘      要：Background Anethole(AN)is an organic antioxidant compound with a benzene ring and is expected to have a positive impact on early embryogenesis in ***,no study has examined the effect of AN on porcine embryonic ***,we investigated the effect of AN on the development of porcine embryos and the underlying *** We cultured porcine in vitro-fertilized embryos in medium with AN(0,0.3,0.5,and 1 mg/mL)for 6 *** at 0.5 mg/mL significantly increased the blastocyst formation rate,trophectoderm cell number,and cellular survival rate compared to the ***-supplemented embryos exhibited significantly lower reactive oxygen species levels and higher glutathione levels than the ***,AN significantly improved the quantity of mitochondria and mitochondrial membrane potential,and increased the lipid droplet,fatty acid,and ATP ***,the levels of proteins and genes related to the sonic hedgehog(SHH)signaling pathway were significantly increased by *** These results revealed that AN improved the developmental competence of porcine preimplantation embryos by activating SHH signaling against oxidative stress and could be used for large-scale production of high-quality porcine embryos.