SnoRNP is essential for thermospermine-mediated development in Arabidopsis thaliana

作     者：Xilong Li Zongyun Yan Meiling Zhang Jiayin Wang Peiyong Xin Shujing Cheng Liquan Kou Xiaoting Zhang Songlin Wu Jinfang Chu Chengqi Yi Keqiong Ye Bing Wang Jiayang Li 

作者机构：State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing)Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China State Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking UniversityBeijing 100871China Key Laboratory of RNA BiologyCAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of SciencesBeijing 100039China Hainan Yazhou Bay Seed LaboratorySanya 572025China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2023年第66卷第1期

页      面：2-11页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the National Natural Science Foundation of China(31788103,32122012,32100221,91940302,31430024) the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24040202) the Chinese Academy of Sciences Youth Innovation Promotion Association(2019099) the CAS Key Technology Talent Program(2017)。 

主　　题：polyamines thermospermine snoRNP pseudouridylation 2’-O-methylation Arabidopsis thaliana 

摘      要：Polyamines have been discovered for hundreds of years and once considered as a class of phytohormones.Polyamines play critical roles in a range of developmental processes.However,the molecular mechanisms of polyamine signaling pathways remain poorly understood.Here,we measured the contents of main types of polyamines,and found that endogenous level of thermospermine(T-Spm)in Arabidopsis thaliana is comparable to those of classic phytohormones and is significantly lower than those of putrescine(Put),spermidine(Spd),and spermine(Spm).We further found a nodule-like structure around the junction area connecting the shoot and root of the T-Spm biosynthetic mutant acl5 and obtained more than 50 suppressors of acl5 nodule structure(san)through suppressor screening.An in-depth study of two san suppressors revealed that NAP57 and NOP56,core components of box H/ACA and C/D snoRNPs,were essential for T-Spm-mediated nodule-like structure formation and plant height.Furthermore,analyses of rRNA modifications showed that the overall levels of pseudouridylation and 2′-O-methylation were compromised in san1 and san2 respectively.Taken together,these results establish a strong genetic relationship between rRNA modification and T-Spm-mediated growth and development,which was previously undiscovered in all organisms.