Remote ischemic conditioning-induced hyperacute and acute responses of plasma proteome in healthy young male adults:a quantitative proteomic analysis

作     者：Siying Song Hao Wu Yunhuan Liu Duo Lan Baolian Jiao Shuling Wan Yibing Guo Da Zhou Yuchuan Ding Xunming Ji Ran Meng 

作者机构：Department of NeurologyXuanwu HospitalCapital Medical UniversityBeijing 100053China Advanced Center of StrokeBeijing Institute for Brain DisordersBeijing 100053China Department of China-America Institute of NeuroscienceXuanwu HospitalCapital Medical UniversityBeijing 100053China Department of NeurosurgeryXuanwu HospitalCapital Medical UniversityBeijing 100053China Department of NeurologyHuadong HospitalFudan UniversityShanghai 200031China Department of NeurosurgeryWayne State University School of MedicineDetroitMI 48201USA 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2023年第136卷第2期

页      面：150-158页

核心收录：

学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：supported by the National Key R&D Program of China(2017YFC1308400) the National Natural Science Foundation(81371289) and the Beijing Natural Science Foundation(7212047) and Capital Medical Development Scientific Research Fund(2022-2-2015) 

主　　题：Healthy male adult Remote ischemic conditioning Quantitative proteomics analysis Beneficial effect 

摘      要：Background: Long-term remote ischemic conditioning (RIC) has been proven to be beneficial in multiple diseases, such as cerebral and cardiovascular diseases. However, the hyperacute and acute effects of a single RIC stimulus are still not clear. Quantitative proteomic analyses of plasma proteins following RIC application have been conducted in preclinical and clinical studies but exhibit high heterogeneity in results due to wide variations in experimental setups and sampling procedures. Hence, this study aimed to explore the immediate effects of RIC on plasma proteome in healthy young adults to exclude confounding factors of disease entity, such as medications and gender. Methods: Young healthy male participants were enrolled after a systematic physical examination and 6-month lifestyle observation. Individual RIC sessions included five cycles of alternative ischemia and reperfusion, each lasting for 5 min in bilateral forearms. Blood samples were collected at baseline, 5 min after RIC, and 2 h after RIC, and then samples were processed for proteomic analysis using liquid chromatography-tandem mass spectrometry method. Results: Proteins related to lipid metabolism (e.g., Apolipoprotein F), coagulation factors (hepatocyte growth factor activator preproprotein), members of complement cascades (mannan-binding lectin serine protease 1 isoform 2 precursor), and inflammatory responses (carboxypeptidase N catalytic chain precursor) were differentially altered at their serum levels following the RIC intervention. The most enriched pathways were protein glycosylation and complement/coagulation cascades. Conclusions: One-time RIC stimulus may induce instant cellular responses like anti-inflammation, coagulation, and fibrinolysis balancing, and lipid metabolism regulation which are protective in different perspectives. Protective effects of single RIC in hyperacute and acute phases may be exploited in clinical emergency settings due to apparently beneficial alterations in plas