Electrofluorochromic imaging analysis of dopamine release from living PC12 cells with bipolar nanoelectrodes array

作     者：Zhaoyan Tian Xiang Qin Fengying Shao Xiuxiu Li Zhi Wang Songqin Liu Yafeng Wu Zhaoyan Tian;Xiang Qin;Fengying Shao;Xiuxiu Li;Zhi Wang;Songqin Liu;Yafeng Wu

作者机构：Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and DeviceJiangsu Province Hi-Tech Key Laboratory for Bio-Medical ResearchState Key Laboratory of BioelectronicsSchool of Chemistry and Chemical EngineeringSoutheast UniversityNanjing 211189China State Key Laboratory of Analytical Chemistry for Life ScienceSchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Wuxi Institute of InspectionTesting and CertificationWuxi 214125China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2023年第34卷第1期

页      面：578-582页

核心收录：

学科分类：081704[工学-应用化学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.22174016,21874018,21627806,21635004) Fundamental Research Funds for the Central Universities(No.2242022K40018) 

主　　题：Au nanoelectrodes array Bipolar nanoelectrodes Dopamine release Electrofluorochromic imaging Real-time monitoring 

摘      要：The coupling of bipolar electrode(BPE)arrays and electrofluorochromic(EFC)imaging has exhibited great abilities in ***,the imaging resolution and analytical performance are hampered by the large size of the electrode and the rapid diffusion of EFC molecules on the electrode ***,to address the challenges,bipolar nanoelectrodes(BPn E)array and in situ immobilization strategy of EFC molecules were *** aluminum oxide(AAO)template-assisted Au nanoelectrodes array with high density was fabricated as BPn E array for high spatial imaging *** electrically polymerizing EFC molecules on the surface of single Au nanoelectrode,the rapid diffusion of EFC molecules on the electrode surface was not only avoided,but also realizing electrofluorescent imaging on an individual *** dopamine(DA)released from living PC12 cells as a model,the proposed strategy exhibited an ultra-high sensitivity for DA analysis with a detection limit of 0.45 nmol/L and the DA release amount from a single cell was calculated to be 0.13 pmol/***,the dynamic change of DA release under the drug stimulation from living PC12 cells could also be monitored.