High-quality Cymbidium mannii genome and multifaceted regulation of crassulacean acid metabolism in epiphytes

作     者：Weishu Fan Zheng-Shan He Mengqing Zhe Jing-Qiu Feng Le Zhang Yiwei Huang Fang Liu Jia-Lin Huang Ji-Dong Ya Shi-Bao Zhang Jun-Bo Yang Andan Zhu De-Zhu Li 

作者机构：Germplasm Bank of Wild SpeciesKunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China Kunming College of Life ScienceUniversity of Chinese Academy of SciencesKunmingYunnan 650201China Key Laboratory for Economic Plants and BiotechnologyKunming Institute of BotanyChinese Academy of SciencesKunmingYunnan 650201China Yuxi Normal UniversityYuxiYunnan 653100China 

出 版 物：《Plant Communications》 (植物通讯（英文）)

年 卷 期：2023年第4卷第5期

页      面：191-207页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：funded by the Strategic Priority Research Program of the Chinese Academy of Sciences(grant no.XDB31000000)to D.-Z.L.and J.-B.Y CAS Pioneer Hundred Talents Program to A.Z the Project for Innovation Team of Yunnan Province(grant no.202105AE160012)to S.-B.Z the Science and Technology Basic Resources Investigation Program of China(grant no.2021FY100200)to J.-B.Y 

主　　题：epiphytes CAM photosynthesis multi-omics phase shifts rhythmic metabolites 

摘      要：Epiphytes with crassulacean acid metabolism(CAM)photosynthesis are widespread among vascular plants,and repeated evolution of CAM photosynthesis is a key innovation for micro-ecosystem ***,we lack a complete understanding of the molecular regulation of CAM photosynthesis in ***,we report a high-quality chromosome-level genome assembly of a CAM epiphyte,Cymbidium mannii(Orchidaceae).The 2.88-Gb orchid genome with a contig N50 of 22.7 Mb and 27192 annotated genes was organized into 20 pseudochromosomes,82.8%of which consisted of repetitive *** expansions of long terminal repeat retrotransposon families have made a major contribution to the evolution of genome size in Cymbidium *** reveal a holistic scenario of molecular regulation of metabolic physiology using high-resolution transcriptomics,proteomics,and metabolomics data collected across a CAM diel *** of rhythmically oscillating metabolites,especially CAM-related products,reveal circadian rhythmicity in metabolite accumulation in *** analysis of transcript and protein level regulation revealed phase shifts during the multifaceted regulation of circadian ***,we observed diurnal expression of several core CAM genes(especially bCA and PPC)that may be involved in temporal fixation of carbon *** study provides a valuable resource for investigating post-transcription and translation scenarios in ***,an Orchidaceae model for understanding the evolution of innovative traits in epiphytes.