The microRNA pathway activation in insect cell model upon Autographa californica multiple nucleopolyhedrovirus infection

作     者：QIAOJIN JIA YUEJUN FU 

作者机构：Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of EducationInstitute of BiotechnologyShanxi UniversityTaiyuan030006China Department of Medical Laboratory ScienceFenyang CollegeShanxi Medical UniversityFenyang032200China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2023年第47卷第3期

页      面：627-645页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：funded by the Natural Science Foundation of Shanxi Province,Grant No.201801D121193 the Central Government Guiding Local Science and Technology Development Fund Project,Grant No.YDZJSX2022A001 

主　　题：microRNAs Spodoptera frugiperda Autographa californica multiple nucleopolyhedrovirus Host-virus interaction 

摘      要：Background:The immune system of insects exerts fundamentally different antiviral mechanisms than ***(miRNAs)play vital roles in developing insect antiviral *** expression profiles of insects changed significantly during baculovirus ***:Differential expression profiles of miRNAs in Spodoptera frugiperda were monitored by next-generation sequencing(NGS)and RT-qPCR during Autographa californica multiple nucleopolyhedrovirus(AcMNPV)*** transcription levels of genes were detected by *** 50%tissue culture infective dose(TCID_(50))endpoint dilution assay was used to determine the proliferation of progeny ***:NGS revealed that 49 miRNAs were differentially expressed in Sf9 cells,and 10 of them were significantly up-or *** RT-qPCR analysis,we observed the similar trends for the expression patterns of significantly differentially expressed miRNAs from ***,the transcription levels of core genes,Exportin5,Dicer1,and Argonaute1,in miRNA biogenesis pathways were significantly increased after AcMNPV *** five selected miRNAs,miR-34-5p could regulate the proliferation of baculovirus progeny virus and energy ***:The miRNAs biogenesis pathway in Sf9 cells plays an important role and may be stimulated to resist AcMNPV *** work provides evidence for the molecular mechanism of baculovirus-insect interaction and offers novel ideas and directions for green pest control technology.