Low-temperature 3D-printed collagen/chitosan scaffolds loaded with exosomes derived from neural stem cells pretreated with insulin growth factor-1 enhance neural regeneration after traumatic brain injury

作     者：Xiao-Yin Liu Yin-He Feng Qing-Bo Feng Jian-Yong Zhang Lin Zhong Peng Liu Shan Wang Yan-Ruo Huang Xu-Yi Chen Liang-Xue Zhou Xiao-Yin Liu;Yin-He Feng;Qing-Bo Feng;Jian-Yong Zhang;Lin Zhong;Peng Liu;Shan Wang;Yan-Ruo Huang;Xu-Yi Chen;Liang-Xue Zhou

作者机构：Department of NeurosurgeryWest China HospitalWest China Medical SchoolSichuan UniversityChengduSichuan ProvinceChina Tianjin Key Laboratory of Neurotrauma RepairPingjin Hospital Brain CenterCharacteristic Medical Center of Chinese People’s Armed Police ForceTianjinChina Department of Respiratory and Critical Care MedicinePeople’s Hospital of Deyang CityAffiliated Hospital of Chengdu College of MedicineDeyangSichuan ProvinceChina Department of Liver Surgery&Liver TransplantationState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengduProvinceChina Department of General Surgerythe Affiliated Hospital of Guizhou Medical UniversityGuiyangGuizhou ProvinceChina The First Affiliated Hospital of Chengdu Medical CollegeChengduSichuan ProvinceChina Department of AnesthesiologyHuashan HospitalFudan UniversityShanghaiChina Institute of Medical Security for Maritime Rights Protection of Characteristic Medical Center of Chinese People’s Armed Police ForceTianjinChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2023年第18卷第9期

页      面：1990-1998页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the National Major Scientific and Technological Special Project for Significant New Drugs Development No.2019ZX09301-147 (to LXZ)。 

主　　题：3D printing angiogenesis chitosan collagen exosomes functional recovery insulin-like growth factor-1 neural regeneration neural stem cells traumatic brain injury 

摘      要：There are various clinical treatments for traumatic brain injury,including surgery,drug therapy,and rehabilitation therapy;howeve r,the therapeutic effects are limited.Scaffolds combined with exosomes represent a promising but challenging method for improving the repair of traumatic brain injury.In this study,we determined the ability of a novel 3D-printed collagen/chitosan scaffold loaded with exosomes derived from neural stem cells pretreated with insulin-like growth factor-1(3D-CC-INEXOS) to improve traumatic brain injury repair and functional recove ry after traumatic brain injury in rats.Composite scaffolds comprising collagen,chitosan,and exosomes derived from neural stem cells pretreated with insulin-like growth fa ctor-1(INEXOS) continuously released exosomes for 2weeks.Transplantation of 3D-CC-INExos scaffolds significantly improved motor and cognitive functions in a rat traumatic brain injury model,as assessed by the Morris water maze test and modified neurological seve rity scores.In addition,immunofluorescence staining and transmission electron microscopy showed that3D-CC-INExos implantation significantly improved the recove ry of damaged nerve tissue in the injured area.In conclusion,this study suggests that transplanted3D-CC-INExos scaffolds might provide a potential strategy for the treatment of traumatic brain injury and lay a solid foundation for clinical translation.