High auxin stimulates callus through SDG8-mediated histone H3K36 methylation in Arabidopsis

作     者：Jun Ma Qiang Li Lei Zhang Sen Cai Yuanyuan Liu Juncheng Lin Rongfeng Huang Yongqiang Yu Mingzhang Wen Tongda Xu Jun Ma;Qiang Li;Lei Zhang;Sen Cai;Yuanyuan Liu;Juncheng Lin;Rongfeng Huang;Yongqiang Yu;Mingzhang Wen;Tongda Xu

作者机构：College of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002China Plant Synthetic Biology CenterFAFU-UCR Joint Center for Horticultural Biology and MetabolomicsHaixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002China Shanghai Center for Plant Stress BiologyCAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Basic Forestry and Proteomics Research CenterHaixia Institute of Science and TechnologyFujian Agricuture and Forestry UniversityFuzhou 350002China Frontiers Science Center for Synthetic Biology(Ministry of Education)Tianin UniversityTianjin 300072China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第12期

页      面：2425-2437页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 

基　　金：the National Natural Science Foundation of China(Grant Nos 32130010,31422008,and 31870256) startup funds from FAFU to T.X.,and FAFU Youth Fund(Grant No.XJQ202016)to J.M. 

主　　题：auxin callus formation epigenetic regulation H3K36 methylation SDG8 TIR1/AFBs 

摘      要：Callus induction,which results in fate transition in plant cells,is considered as the first and key step for plant regeneration.This process can be stimulated in different tissues by a callus-inducing medium(CIM),which contains a high concentration of phytohormone auxin.Although a few key regulators for callus induction have been identified,the multiple aspects of the regulatory mechanism driven by high levels of auxin still need further investigation.Here,we find that high auxin induces callus through a H3 K36 histone methylation-dependent mechanism,which requires the methyltransferase SET DOMAIN GROUP 8(SDG8).During callus induction,the increased auxin accumulates SDG8 expression through a TIR1/AFBs-based transcriptional regulation.SDG8 then deposits H3 K36 me3 modifications on the loci of callus-related genes,including a master regulator WOX5 and the cell proliferation-related genes,such as CYCB1.1.This epigenetic regulation in turn is required for the transcriptional activation of these genes during callus formation.These findings suggest that the massive transcriptional reprogramming for cell fate transition by auxin during callus formation requires epigenetic modifications including SDG8-mediated histone H3 K36 methylation.Our results provide insight into the coordination between auxin signaling and epigenetic regulation during fundamental processes in plant development.