Pharmacodynamics of frigid zone plant Taxus cuspidata *** *** skin melanin deposition,oxidation,inflammation and allergy

作     者：Yang Shi Ling Liu Heyang Sun Chen Chen Jing Feng Yongchao Chen Yuan Lin Philipp Kopylov Qi Wang Yong Zhang Yang Shi;Ling Liu;Heyang Sun;Chen Chen;Jing Feng;Yongchao Chen;Yuan Lin;Philipp Kopylov;Qi Wang;Yong Zhang

作者机构：Department of Pharmacology(the State-Province Key Laboratories of Biomedicine Pharmaceutics of ChinaKey Laboratory of Cardiovascular ResearchMinistry of Education)College of PharmacyHarbin Medical UniversityHarbin 150081China Department of Medicinal chemistry and natural medicinal chemistryCollege of PharmacyHarbin Medical UniversityHarbin 150081China Research Unit of Noninfectious Chronic Diseases in Frigid ZoneChinese Academy of Medical Sciences2019RU070Harbin 150081China Institute of Metabolic DiseaseHeilongjiang Academy of Medical ScienceHarbin 150086China Department of Preventive and Emergency CardiologySechenov First Moscow State Medical UniversityMoscow 119991Russia 

出 版 物：《Frigid Zone Medicine》 (寒地医学（英文）)

年 卷 期：2023年第3卷第1期

页      面：42-52页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of Heilongjiang Province-Outstanding Youth Project(No.YQ2020H008) 

主　　题：Taxus cuspidata S.et Z. skin melanin deposition oxidation inflammation allergy 

摘      要：Background:Taxus cuspidata *** *** a precious species of frigid zone plant belonging to the Taxaceae family,which possesses anticancer,anti-inflammatory,hypoglycemic,and antibacterial pharmacological *** taxane extracted from Taxus chinensis has been reported to elicit antioxidant activities,whether Taxus cuspidata *** *** skin-protective actions against injuries remained *** study aims to explore the pharmacological effects of three Taxus extracts on skin melanin deposition,oxidation,inflammation,and allergy so as to provide new ideas for the prevention and treatment of various diseases related to skin ***:Skin melanin deposition was evaluated by measuring melanin content in the skin of guinea pigs by alkali lysis *** capacity was evaluated by measuring superoxide dismutase(SOD)concentration and glutathione(GSH)content in skin tissue homogenates of Kunming mice by SOD assay kit and micro reduced GSH assay *** quantitative real-time polymerase chain reaction(qRT-PCR)and western blotting were used to examine the levels of both SOD and recombinant glutathione peroxidase 4(GPX4).Skin inflammation was evaluated by xylene-induced ear swelling test and egg-white-induced paw swelling test in *** a mouse model of skin allergy induced by 4-aminopyridine(4-AP),allergy was determined by licking body counts and histamine concentrations in tissue homogenates using enzyme-linked immunosorbent assay(ELISA)*** proinflammatory factors tumor necrosis factor(TNF)-αand interleukin(IL)-1βwere measured by *** and eosin(HE)staining was conducted to assess the degree of skin ***:All three Taxus extracts including Taxus chinensis essential oil,Taxus chinensis extract and Taxus chinensis extract compound reduced the melanin deposits in the back skin relative to the non-treated control animals,of which Taxus chinensis essential oil produced the greatest *** contrast,the three Taxus extracts elev