Plant cultivar determined bacterial community and potential risk of antibiotic resistance gene spread in the phyllosphere

作     者：Xiaoting Fan Jianqiang Su Shuyidan Zhou Xinli An Hu Li Xiaoting Fan;Jianqiang Su;Shuyidan Zhou;Xinli An;Hu Li

作者机构：Key Laboratory of Urban Environment and HealthNingbo Observation and Research StationInstitute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2023年第127卷第5期

页      面：508-518页

核心收录：

学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the National Key Research and Development Plan from Ministry of Science and Technology of the People’s Republic of China(No.2020YFC1806902) the Alliance of International Science Organizations(No.ANSO-PA-2020-18) 

主　　题：Cilantro Antibiotic resistance genes(ARGs) Horizontal gene transfer(HGT) Mobile genetic elements(MGEs) Pathogenic bacteria 

摘      要：The global increased antibiotic resistance level in pathogenic microbes has posed a significant threat to human *** vegetables have been recognized to be an important vehicle of antibiotic resistance genes(ARGs)from environments to human *** ARGs have been indicated to be changed with plant species,yet the influence of plant cultivar on the phyllospheric resistome is still ***,we detected the ARGs and bacterial communities in the phyllosphere of two cultivars of cilantros and their corresponding soils using high-throughput quantitative PCR technique and bacterial 16S rRNA gene-based high-throughput sequencing,*** further identified the potential bacterial pathogens and analyzed the effects of plant cultivar on ARGs,mobile genetic elements(MGEs),microbiome and potential bacterial *** results showed that the cultivars did not affect the ARG abundance and composition,but significantly shaped the abundance of MGEs and the composition structure of bacteria in the *** relative abundance of potential bacterial pathogenswas significantly higher in the phyllosphere than that in *** test showed that the ARG patterns were significantly correlated to the patterns of potential bacterial *** results suggested that the horizontal gene transfer of ARGs in the phyllosphere might be different between the two cultivars of cilantro and highlighted the higher risk of phyllospheric microorganisms compared with those in *** findings extend our knowledge on the vegetable microbiomes,ARGs,and potential pathogens,suggesting more agricultural and hygiene protocols are needed to control the risk of foodborne ARGs.