Variability in fecal metabolome depending on age, PFBS pollutant, and fecal transplantation in zebrafish: A non-invasive diagnosis of health

作     者：Baili Sun Mengyuan Liu Lizhu Tang Xiangzhen Zhou Chenyan Hu Lianguo Chen Baili Sun;Mengyuan Liu;Lizhu Tang;Xiangzhen Zhou;Chenyan Hu;Lianguo Chen

作者机构：State Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of SciencesBeijing 100049China School of Chemistry and Environmental EngineeringWuhan Institute of TechnologyWuhan 430072China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2023年第127卷第5期

页      面：530-540页

核心收录：

学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(No.22006159) the Natural Science Foundation of Hubei Province,China(No.2021CFA086) the Innovative Team Program of Natural Science Foundation of Hubei Province,China(No.2021CFA032) 

主　　题：Fecal metabolome Aging microbiota Perfluorobutanesulfonate(PFBS) Fecal transplantation Non-invasive diagnosis Zebrafish 

摘      要：To protect the wellbeing of research animals,certain non-invasive measures are in increasing need to facilitate an early diagnosis of health and *** this study,feces specimen was collected from adult zebrafish to profile the metabolome *** in fecal metabolite composition was also distinguished as a result of aging,perfluorobutanesulfonate(PFBS)toxicant,and fecal *** results showed that zebrafish feces was very rich in a diversity of metabolites that belonged to several major classes,including lipid,amino acid,carbohydrate,vitamin,steroid hormone,and *** metabolites had functional implications to multiple physiological activities,which were characterized by the enrichment of digestion,absorption,endocrine,and neurotransmission *** high richness and functional involvement of fecal metabolites pinpointed feces as an abundant source of diagnostic *** comparison between young and aged zebrafish,fundamental modifications of fecal metabolomes were caused by aging progression,centering on the neuroactive ligand-receptor interaction *** of aged zebrafish to PFBS pollutant also significantly disrupted the metabolomic structure in *** special concern were the changes in fecal hormone intermediates after PFBS exposure,which was concordant with the in vivo endocrine disrupting effects of ***,itwas intriguing that transplantation of young zebrafish feces efficientlymitigated the metabolic perturbation of PFBS in aged recipients,highlighting the health benefits of therapeutic strategies based on gut microbiota *** summary,the present study provides preliminary clues to evidence the non-invasive advantage of fecal metabolomics in the early diagnosis and prediction of physiology and toxicology.