A Novel Method to Improve the Physical Property and Biocompatibility of Decellularized Heart Valve Scaffold with Sericin and Polydopamine

作     者：Peng Bai Gangcheng Kong Weihua Qiao Yu Song Yixuan Wang Jiawei Shi Nan Zhang Chungeng Liu Chong Chu Tixiusi Xiong Ying Zhou Cuifen Lu Lin Wang Nianguo Dong Peng Bai;Gangcheng Kong;Weihua Qiao;Yu Song;Yixuan Wang;Jiawei Shi;Nan Zhang;Chungeng Liu;Chong Chu;Tixiusi Xiong;Ying Zhou;Cuifen Lu;Lin Wang;Nianguo Dong

作者机构：Department of Cardiovascular SurgeryUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430032China Department of Hepatobiliary and Pancreatic SurgeryThe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China Research Center for Tissue Engineering and Regenerative MedicineUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of EndocrinologyUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430032China Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and MinistryHubei UniversityWuhan 430062China Department of Clinical LaboratoryUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China 

出 版 物：《Journal of Bionic Engineering》 (仿生工程学报（英文版）)

年 卷 期：2022年第19卷第4期

页      面：1109-1123页

核心收录：

学科分类：09[农学] 0906[农学-兽医学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0811[工学-控制科学与工程] 

基　　金：supported by the National Key Research and Development Program of China Stem Cell and Translational Research(2016YFA0101103) the National Natural Science Foundation of China(grant numbers 81930052,81901904,82000367,82001701). 

主　　题：Decellularized heart valve Sericin Polydopamine Biocompatibility 

摘      要：Cardiac valve replacement is an effective method to treat valvular heart disease.Artificial valves used routinely in clinic still have defects.In our study,we explored a novel method to modify the performance of Decellularized Heart Valve(DHV)scaffold.The decellularized porcine aortic valve was prepared using sequential hydrophile and lipophile solubilization method.The sericin was extracted from silk fibroin-deficient silkworm cocoon by lithium bromide method.First,DHV was immersed in sericin solution to produce the sericin–DHV composite scaffold.Then,we modified the DHV by making a Polydopamine(PDA)coating on the DHV first and then binding the sericin.The physical properties and biological compatibility of our composite scaffold were assessed in vitro and in vivo.Sericin were successfully prepared,combined to DHV and improved its biocompatibility.PDA coating further promoted the combination of sericin on DHV and improved the physical properties of scaffolds.The decay rate of our modified valve scaffold was decreased in vivo and it showed good compatibility with blood.In conclusion,our modification improved the physical properties and biocompatibility of the valve scaffold.The combination of PDA and sericin promoted the recellularization of decellularized valves,showing great potential to be a novel artificial valve.