Wenshen Jianpi recipe(温肾健脾方) induced immune reconstruction and redistribution of natural killer cell subsets in immunological non-responders of human immunodeficiency virus/acquired immune deficiency syndrome:a randomized controlled trial

作     者：TAO Zhuang WANG Jian CHEN Xin LI Yonghong YAN Yuguang ZHANG Ao ZOU Wen LIU Ying TAO Zhuang;WANG Jian;CHEN Xin;LI Yonghong;YAN Yuguang;ZHANG Ao;ZOU Wen;LIU Ying

作者机构：Research Center of AIDS Treatment with Traditional Chinese MedicineChina Academy of Chinese Medical SciencesBeijing 100700China Institute of Basic Theory for Chinese MedicineChina Academy of Chinese Medical SciencesBeijing 100700China Department of Integrated Traditional Chinese and Wetern MedicineKunming Hospital of Traditional Chinese MedicineKunming 650000China Department of InfectionNo.6 People's Hospital of Shenyang CityShenyang 110006China University of Chinese Academy of SoCIal SCIencesBeijing100102China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第5期

页      面：795-802页

核心收录：

学科分类：100506[医学-中医内科学] 1005[医学-中医学] 1002[医学-临床医学] 10[医学] 

基　　金：Supported by National Science and Technology Major Project of China During the 13th Five-year Plan Period:New Adjuvant Strategies of Traditional Chinese Medicine on Immune Reconstruction for Longterm ART-treated People Living with HIV(2017ZX10205501) Effects of Traditional Chinese Medicine on Intestinal Bacterial Community Structure of AIDS Patients(2017ZX10205501-001-010) National Natural Science Foundation of China:Immune Mechanism of Immunological Non-responders of HIV/AIDS Treated with Traditional Chinese Medicine from Immune Activation and Toll like Receptor Signal Pathway(No.81503571)。 

主　　题：HIV acquired immunodeficiency syndrome killer cells natural immunological non-responders antiretroviral therapy Wenshen Jianpi recipe immune reconstruction randomized controlled trial 

摘      要：OBJECTIVE:To evaluate the effects of the Wenshen Jianpi recipe(温肾健脾方,WJR)on immune reconstruction and natural killer(NK)cells in immunological non-responders(INRs)of people living with human immunodeficiency virus(HIV)(PLWH)and propose new therapeutic strategies for HIV.METHODS:Based on Traditional Chinese Medicine treatment principle“invigorating Qi and warming Yang in the spleen and kidneys,WJR combined with antiretroviral therapy(ART)therapy was performed in a randomized,double-blind,placebo-controlled study of 60 patients with non-responders.The randomized process was executed by the Clinical Evaluation Center of China Academy of Chinese Medical Sciences.Sixty patients who met the inclusion criteria obtained random numbers(that is the drug number)was randomly divided into a treatment group and a placebo control group according to a 1∶1 ratio.CD4+T cell counts and natural killer(NK)cells counts were evaluated at baseline and 12-week,24-week follow-ups.RESULTS:Four participants received random numbers and did not enter the group due to the patient’s own reasons.A total of 56 patients were enrolled,including 28 in the treatment group and 28 in the control group.CD4+T cell counts in the treatment group were significantly increased at week 24(P=0.010.05),which were significantly higher than those in the control group(P=0.010.05).Although no significant differences were observed between two groups,the CD56bri CD16-NK cell counts in the treatment group were significantly increased after duration.and CD56dim CD16+NK cell counts in the treatment group were significantly higher than those in the control group after 24 weeks of treatment(P=0.0250.05).As compared with the control group,the treatment group had significantly lower CD56neg CD16+NK cell counts after 24 weeks of treatment(P=0.0230.05).CONCLUSIONS:WJR promotes the immune reconstruction of INRs and redistribution of NK cell subsets,notably decreasing CD56neg CD16+NK cell counts in INRs.However,the redistribution of NK cell subsets is not beneficial for immune reconstruction in INRs.Further large-scale RCTs are required to evaluate the effect of WJR on immune recovery in INRs and decipher the underlying mechanism.