Point-of-care testing for lysine concentration in swine serum via blue-emissive carbon dot-entrapped microfluidic chip

作     者：Chizhu Ding Xiang Chen Xiaoyu Chen Yue Liu Menglin Xia Ziyi He Qinshu Kang Xianghua Yan Chizhu Ding;Xiang Chen;Xiaoyu Chen;Yue Liu;Menglin Xia;Ziyi He;Qinshu Kang;Xianghua Yan

作者机构：State Key Laboratory of Agricultural MicrobiologyHubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of ScienceCollege of Animal Sciences and TechnologyCollege of Veterinary MedicineHuazhong Agricultural UniversityWuhan430070China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第12卷第1期

页      面：236-244页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：This study was supported by the National Natural Science Foundation of China(32172782)to C.Z.Ding the National Natural Science Foundation of China(22104041)to Z.Y.He 

主　　题：Lysine Swine serum Carbon dots Microfluidics Point-of-care testing 

摘      要：Lysine is one of the essential amino acids and plays a vital role in the growth,development and health of *** lysine concentration is a direct indication of lysine status;however,current methods can not satisfy the demands for rapid and on-site lysine concentration measurement of swine ***,we developed blue-emissive nitrogen-doped carbon dots as a fluorescence probe for the determination of lysine with high fluorescence quantum yield,stability,sensitivity and *** carbon dots were entrapped within hydrogel microstructures to fabricate microfluidic chips for rapid assay for lysine *** further developed an imaging attachment to integrate the microfluidic chip and a smartphone into a portable point-of-care testing *** platform requires only 3μL sample and has a linear detection range of 25 to 300μmol/L with a limit of detection less than 16μmol/L,which covers the normal range of lysine concentration in swine *** tested lysine concentration in swine serum using this platform with high accuracy,low sample consumption,and within 3 ***,these results may provide a rapid and portable platform for dynamic monitoring of swine lysine status and contribute to precise feed formula modulation with low-protein diet strategy.