Untargeted and targeted mass spectrometry reveal the effects of theanine on the central and peripheral metabolomics of chronic unpredictable mild stress-induced depression in juvenile rats
作者机构：Laboratory of Pharmaceutical AnalysisSchool of PharmacyZhejiang Pharmaceutical UniversityNingboZhejiang315100China Laboratory of Pharmaceutical AnalysisSchool of PharmacyShenyang Pharmaceutical UniversityShenyang110016China
出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)
年 卷 期：2023年第13卷第1期
页 面：73-87页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 100704[医学-药物分析学] 10[医学]
主 题：L-theanine Depression Metabolomics Liquid chromatography-mass spectrometry
摘 要：L-theanine has been shown to have a therapeutic effect on ***,whether L-theanine has an excellent preventive effect on depression in children and adolescents and what its mechanism is have not been well *** the complexity of the pathogenesis of depression,this study investigated the preventive effect and mechanism of L-theanine on depression in juvenile rats by combining serum and hippocampal metabolomic *** tests,hippocampal tissue sections,and serum and hippocampal biochemical indexes were studied,and the results confirmed the preventive effect of *** reversed-phase liquid chromatography-quadrupole-time-of-flight mass spectrometry and targeted hydrophilic interaction liquid chromatography-triple quadrupole mass spectrometry were developed to analyze the metabolism changes in the serum and hippocampus to screen for potential biomarkers related to L-theanine *** results suggested that 28 abnormal metabolites in the serum and hippocampus that were considered as potential biomarkers returned to nearnormal levels after L-theanine *** biomarkers were involved in various metabolic pathways,mainly including amino acid metabolism and lipid *** levels of amino acids and neurotransmitters in the phenylalanine,tryptophan,and glutamic acid pathways were significantly reduced after L-theanine administration compared with chronic unpredictable mild stress-induced *** summary,L-theanine had a significant preventive effect on depression and achieved its preventive results on depression by regulating various aspects of the body,such as amino acids,lipids,and *** research systematically analyzed the mechanism of L-theanine in preventing depression and laid the foundation for applying L-theanine to prevent depression in children and adolescents.
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