Effect of cold stratification on the temperature range for germination of Pinus koraiensis

作     者：Yuan Song Xinghuan Li Mingyi Zhang Guowei Xia Chao Xiong Yuan Song;Xinghuan Li;Mingyi Zhang;Guowei Xia;Chao Xiong

作者机构：College of Eco-Environmental EngineeringGuizhou Minzu UniversityGuiyangPeople’s Republic of China Qingyuan Forest CERNChinese Academy of SciencesShenyang 110016People’s Republic of China Karst Environmental Geological Hazard Prevention Laboratory of Guizhou Minzu UniversityGuiyangPeople’s Republic of China Guiyang Institute of Information Science and TechnologyGuiyangPeople’s Republic of China 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2023年第34卷第1期

页      面：221-231页

核心收录：

学科分类：0710[理学-生物学] 0907[农学-林学] 07[理学] 071007[理学-遗传学] 

基　　金：the National Natural Science Foundation of China(No.31901300) Natural Science Foundation of Guizhou Province China(No.(2019)1165) Science and Technology Foundation of Guizhou Province China(No.137,No.133)。 

主　　题：Cold Stratification temperatures Pinus koraiensis Seed germination Germination temperature range 

摘      要：Germination at low spring temperatures may offer a competitive advantage for the growth and survival of plant species inhabiting temperate forest ecosystems.Pinus koraiensis is a dominant species in temperate forests of northeastern China.Its seeds exhibit primary morphophysiological dormancy following dispersal in autumn,limiting natural or artificial regeneration:direct seeding and planting seedlings in spring.The aim of this study was to determine the optimum cold stratification temperature that induces germination to increase towards lower temperatures.Seeds from two populations(Changbaishan and Liangshui)were cold stratified at 0,5 and 10℃.Germination to incubation temperatures(10/5,20/10,25/15 and 30/20℃;14/10 h day/night)were determined after 2 and 4 weeks,and 5.5 and6.5 months of cold stratification.After 5.5 months,approximately 68-91%of seeds from both populations germinated at incubation temperatures of 25/15℃and 30/20℃,regardless of cold stratification temperatures.When the cold stratification temperature was reduced to 0℃and the period increased to 6.5 months,germination at 10/5℃significantly improved,reaching 37%and 64%for the Changbaishan and Liangshui populations,respectively.After 6.5 months of cold stratification,there was a significant linear regression between cold stratification temperatures and germination at10/5℃.The range in temperatures allowing for germination gradually expanded to include lower temperatures with decreasing cold stratification temperatures from 10 to 5℃and further to 0℃.