Molecular mechanism analysis of Miao medicine Jinwujiangu decoction(金乌健骨方) in treating osteoarthritis based on a network pharmacology approach

作     者：JIANG Zong YAO Xiaoling MA Wukai TANG Fang JIANG Zong;YAO Xiaoling;MA Wukai;TANG Fang

作者机构：Department of Rheumatologythe Second Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyang 550002China Department of Traditional Chinese MedicineLonghua District Central HospitalShenzhen 518110China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第4期

页      面：576-585页

核心收录：

学科分类：1006[医学-中西医结合] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：Supported by the Program of the National Natural Science Foundation of China:Molecular Mechanism of Miao Medicine Jinwu Jiangu Decoction in the Treatment of Knee Osteoarthritis Based on Axin-Wnt Signal Regulation Gene Molecular Mechanism of Miao Medicine Wuteng Paste in the Treatment of Knee Osteoarthritis Based on WntNotch Signaling(No.81760866,81760884) Special Project of TCM Modernization of the National Key Research and Development Plan of China:Research on Inheritance of Ethnic Medicine(2017YFC-1703904) the Science and Technology Program of Guizhou Province:Study on the Mechanism of Wenjingtongluo Formula Regulating Osteoarthritis Model Rabbits based on Axin Regulatory Factors(Qian Ke He Foundation1011),the Science and Technology Program of Guizhou Province:the Hundred Talent Project of Guizhou Province High-Level Innovation Talent Training Plan(Qian Ke He Platform Talent5650) Construction of Medical Platform of Traditional Chinese Medicine(Ethnic Medicine)in the Treatment of Rheumatism in Guizhou Province(Qian Ke He Platform Talent5707) Clinical Research Center of Rheumatology and Immunology in Guizhou Province(Qian Ke He Platform Talent2202) 

主　　题：osteoarthritis network pharmacology targets Jinwujiangu decoction 

摘      要：OBJECTIVE:To investigate molecular mechanisms of Jinwujiangu decoction(金乌健骨方,JWJG)in treating osteoarthritis(OA)using network pharmacology analysis.METHODS:Principal active compounds of JWJG were screened out via the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and BATMAN-TCM,and potential targets for OA treatment were identified through Online Mendelian Inheritance in Man(OMIM)and Gene Cards databases.The JWJG network diagrams of both principal chemical compound-action targets and OA treatment target-OA disease were constructed applying the Cytoscape 3.7.2 software.The diagram of protein-protein interaction network was plotted for core analysis.Meanwhile,the common targets and relevant signaling pathways involved in both networks were analyzed using the Gene Ontology function analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment.The predicted results were ultimately verified through animal experiments.RESULTS:Effects of JWJG were indicated in acting on key targets interleukin-6,insulin,protein kinase B,glyceraldehyde-3-phosphate dehydrogenase,and mitosis-specific MRE11-RAD50-NBS1 associated protein by regulating signaling pathways of phosphoinositide 3-kinase-protein kinase B,mitogen-activated protein kinases,tumor necrosis factor,and colorectal cancer.Meanwhile,it inhibited the over-activation of signaling pathways and the release of inflammatory factors in OA treatment.Following a signaling pathway analysis utilizing network pharmacology technique,it was demonstrated that JWJG could treat OA through the Wnt/β-catenin signaling pathway verified by animal experiments.CONCLUSIONS:The present study preliminarily analyzed the pharmacological mechanism of JWJG in treating OA on a network pharmacology approach and provided a great theoretical significance for clinical application.