Single-cell RNA-seq analysis of testicular somatic cell development in pigs

作     者：Lingkai Zhang Ming Guo Zidong Liu Ruifang Liu Yi Zheng Taiyong Yu Yinghua Lv Hongzhao Lu Wenxian Zeng Tao Zhang Chuanying Pan Lingkai Zhang;Ming Guo;Zidong Liu;Ruifang Liu;Yi Zheng;Taiyong Yu;Yinghua Lv;Hongzhao Lu;Wenxian Zeng;Tao Zhang;Chuanying Pan

作者机构：College of Animal Science and TechnologyNorthwest A&F UniversityYanglingShaanxi 712100China College of Chemistry and PharmacyNorthwest A&F UniversityYanglingShaanxi 712100China School of Biological Science and EngineeringShaanxi University of TechnologyHanzhongShaanxi 723001China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2022年第49卷第11期

页      面：1016-1028页

核心收录：

学科分类：0710[理学-生物学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported in part by the National Natural Science Foundation of China(31772605) Research Project of Shaanxi Science and Technology Department(2020NY-003)to Tao Zhang。 

主　　题：Pigs scRNA-seq Testis somatic cells Puberty 

摘      要：Spermatogenesis is the process by which diploid male germ cells propagate and differentiate into haploid flagellated spermatozoa.This highly complicated process is dependent on testicular somatic cells maturation.While the role of these somatic cells in spermatogenesis is relatively well established,knowledge about their development and maturation,particularly at the molecular level,is limited.In this study,we profiled the testicular single-cell transcriptomes of Guanzhong black pigs at the age of 7,30,60,90,and 150 days.Five types of Sertoli cells,five types of Leydig cells,and four types of peritubular myoid cells were identified.Histological analysis revealed the changes in proliferation levels and marker gene expressions,and the prion-like protein gene(PRND)was identified as a novel marker for Sertoli cells.Additionally,integrated analyses of porcine and human datasets revealed similarities between human and pig testicular somatic cells.Overall,the data obtained in this study contribute to the understanding of testicular development in pigs as a model species.