iTRAQ proteomics reveals the regulatory response to Xanthomonas campestris *** in resistant *** pepper genotypes

作     者：Fei Wang Shenghua Gao Juntawong Niran Ning Li Yanxu Yin Chuying Yu Chunhai Jiao Minghua Yao Fei Wang;Shenghua Gao;Juntawong Niran;Ning Li;Yanxu Yin;Chuying Yu;Chunhai Jiao;Minghua Yao

作者机构：Hubei Academy of Agricultural SciencesHubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic ImprovementCash Crops Research InstituteHubei Academy of Agricultural SciencesWuhanHubei 430070China Kasetsart UniversityBangkok 10900Thailand 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2022年第8卷第6期

页      面：747-756页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by grants of the National Key R&D Program of China (Grants Nos.2016YFE0205500 and 2017YFD0101903) the earmarked fund for China Agriculture Research System (Grant No.CARS-23-G28) the China Postdoctoral Science Foundation (Grant No.2017M620305) Natural Science Foundation of Hubei Province (Grant No.2020CFA010) Youth Fund of Hubei Academy of Agricultural Sciences (Grant No.2021NKYJJ04) 

主　　题：Pepper Proteomics analysis Bacterial spot Xanthomonas campestris pv.vesicatoria 

摘      要：Bacterial spot(BS)is a severe bacterial disease induced by Xanthomonas campestris ***(Xcv),a pathogen that causes serious damage to pepper growth and *** is therefore important to study the mechanisms of pepper resistance to Xcv and to breed and promote Xcvresistant pepper ***,studies of the responses to Xcv infection in peppers at the protein level are ***,we examined Xcv-induced proteomic changes in leaves of the BS susceptible bell pepper ECW and the resistant bell pepper VI037601 using the isobaric tags for relative and absolute quantitation(iTRAQ)-based protein labeling technology.A total of 6,120 distinct proteins were identified,and there were 1,289 significantly differentially accumulated proteins(DAPs)in ECW and VI037601 leaves after Xcv *** these,339(250up-and 89 down-regulated)and 479(300 up-and 179 down-regulated)DAPs were specifically identified in ECW and VI037601,respectively,with 459(364 up-and 95 down-regulated)similarly expressed DAPs being shared by ECW and *** on bioinformatics analysis,many defense-associated proteins were identified as up-regulated in ECW and VI037601,especially the proteins involved in plant-pathogen interaction,phenylpropanoid biosynthesis,protein processing in the endoplasmic reticulum,and MAPK signaling ***,we evaluated transcript levels of six differentially expressed genes from the iTRAQ results by q *** analysis revealed transcriptional changes that were consistent with the changes at the protein *** study will provide a valuable resource for understanding the molecular basis of pepper resistance to Xcv infection and for improving the disease resistance of pepper cultivars.