NMR Analysis of the Structure of Leucine Enkephalin in Solution

作     者：罗雪春 涂光忠 张日清 赵南明 

作者机构：Department of Biological Science and Biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing 100084 

出 版 物：《Tsinghua Science and Technology》 (清华大学学报（自然科学版（英文版）)

年 卷 期：1998年第3卷第3期

页      面：59-61页

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 07[理学] 

主　　题：D NMR solution structure Leucine Enkephlin 

摘      要：To investigate the interaction between membranes and polypeptides, the structure of the polypeptide must be known first. Enkephalin, a natural ligand for opiate receptors is composed of the pentapeptides H 2N Tyr Gly Gly Phe Met OH and H 2N Tyr Gly Gly Phe Leu OH. Two dimensional 1 H NMR techniques can be used to determine the structure of Leucine Enkephalin in solution and to build a foundation for research on the interaction between Enkephalin and the membrane. Complete proton resonance assignments were obtained using 2 D COSY, TOCSY, and NOESY techniques. 